Arginine side chain interactions and the role of arginine as a gating charge carrier in voltage sensitive ion channels

Scientific Reports
Craig T ArmstrongChristopher E Dempsey

Abstract

Gating charges in voltage-sensing domains (VSD) of voltage-sensitive ion channels and enzymes are carried on arginine side chains rather than lysine. This arginine preference may result from the unique hydration properties of the side chain guanidinium group which facilitates its movement through a hydrophobic plug that seals the center of the VSD, as suggested by molecular dynamics simulations. To test for side chain interactions implicit in this model we inspected interactions of the side chains of arginine and lysine with each of the 19 non-glycine amino acids in proteins in the protein data bank. The arginine guanidinium interacts with non-polar aromatic and aliphatic side chains above and below the guanidinium plane while hydrogen bonding with polar side chains is restricted to in-plane positions. In contrast, non-polar side chains interact largely with the aliphatic part of the lysine side chain. The hydration properties of arginine and lysine are strongly reflected in their respective interactions with non-polar and polar side chains as observed in protein structures and in molecular dynamics simulations, and likely underlie the preference for arginine as a mobile charge carrier in VSD.

References

Aug 5, 1988·Journal of Molecular Biology·N ThankiJ M Goodfellow
Jan 14, 1994·Journal of Molecular Biology·M M Flocco, S L Mowbray
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·J P Gallivan, D A Dougherty
Dec 11, 1999·Nucleic Acids Research·H M BermanP E Bourne
Apr 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·P E MasonJ M Cruickshank
Aug 13, 2003·Bioinformatics·Guoli Wang, Roland L Dunbrack
Jan 12, 2005·Biochemistry·Christopher E DempseyPhilip E Mason
Oct 6, 2005·Neuron·Christopher A Ahern, Richard Horn
May 2, 2007·Proceedings of the National Academy of Sciences of the United States of America·Fabiana V CamposFrancisco Bezanilla
Jul 17, 2007·Progress in Biophysics and Molecular Biology·Pinak Chakrabarti, Rajasri Bhattacharyya
Mar 29, 2008·Protein Science : a Publication of the Protein Society·Michael J HarmsBertrand García-Moreno
Feb 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Woon Ki LimS Walter Englander
Oct 31, 2009·Journal of the American Chemical Society·Philip E MasonJohn W Brady
Apr 3, 2010·Science·Xiao TaoRoderick MacKinnon
Feb 3, 2011·Proteins·Jason E DonaldWilliam F DeGrado
Mar 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Lucie DelemotteWerner Treptow
Apr 6, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jérôme J Lacroix, Francisco Bezanilla
Nov 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Michael J HarmsBertrand García-Moreno
Apr 14, 2012·Science·Morten Ø JensenDavid E Shaw
Jun 21, 2012·Biophysical Journal·J Alfredo FreitesDouglas J Tobias
Aug 28, 2013·The Journal of General Physiology·Yen May ChengTom W Claydon
Sep 7, 2013·The Journal of Physical Chemistry. B·Libo LiToby W Allen
Feb 4, 2014·Nature Structural & Molecular Biology·Qufei LiEduardo Perozo
Mar 4, 2014·Nature Structural & Molecular Biology·Kohei TakeshitaAtsushi Nakagawa
Apr 8, 2014·The Journal of Physical Chemistry. B·Karl T DebiecLillian T Chong
Apr 30, 2014·The Journal of General Physiology·Stephan A PlessChristopher A Ahern
May 2, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jérôme J LacroixFrancisco Bezanilla
Jul 30, 2014·The Journal of General Physiology·Tamer M Gamal El-DinWilliam A Catterall
Nov 25, 2014·Biophysical Journal·Charlotte K ColensoChristopher E Dempsey
Mar 27, 2015·Protein Science : a Publication of the Protein Society·Carolyn A FitchLawrence P McIntosh
Oct 9, 2012·Journal of Chemical Theory and Computation·Maria M ReifChris Oostenbrink

❮ Previous
Next ❯

Citations

Feb 24, 2017·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Bo XuMusahid Ahmed
May 21, 2017·Interdisciplinary Sciences, Computational Life Sciences·M Kalim A KhanJamal M Arif
Nov 30, 2016·Angewandte Chemie·Yuichi YoshimuraFrans A A Mulder
Nov 18, 2017·Physical Chemistry Chemical Physics : PCCP·Juan Ramón Avilés-MorenoBruno Martínez-Haya
Nov 15, 2017·Proceedings of the National Academy of Sciences of the United States of America·Justin G FedorJudy Hirst
Jun 27, 2018·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Juan Ramón Avilés-MorenoBruno Martínez-Haya
Aug 8, 2018·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Harold W Mackenzie, D Flemming Hansen
Oct 18, 2019·Biotechnology Progress·Christa Meingast, Caryn L Heldt
Nov 27, 2019·Pharmacology Research & Perspectives·James R FosterAndrew J Brown
Oct 27, 2017·Proceedings of the National Academy of Sciences of the United States of America·Giulio TeseiMikael Lund
Dec 19, 2018·Proceedings of the National Academy of Sciences of the United States of America·Erin E McClure CarrollJoao H F Pedra
Jun 7, 2020·Biomolecules·Kristen ScopinoMichael P Weir
Jan 23, 2018·Physical Chemistry Chemical Physics : PCCP·Juan Ramón Avilés-MorenoBruno Martínez-Haya
Sep 30, 2020·The Journal of Antimicrobial Chemotherapy·Cristina F MallaMaría M Tavío
Oct 7, 2020·Viral Immunology·Camilla M DonnellyJade K Forwood
Oct 3, 2020·Parasitology·Cibele Nicolaski PedronVani Xavier Oliveira
Nov 27, 2020·Biotechnology and Bioengineering·Vitali StanevichJohn Schreffler
Jan 15, 2021·Journal of Peptide Science : an Official Publication of the European Peptide Society·Cibele Nicolaski PedronVani Xavier Oliveira Junior
Feb 13, 2021·International Journal of Molecular Sciences·Kristen ScopinoMichael P Weir
Jun 24, 2021·FEMS Microbiology Reviews·Victor M HernándezMichael F Dunn
May 28, 2019·The Journal of Physical Chemistry Letters·Philip E MasonElise Duboué-Dijon
May 31, 2019·The Journal of Physical Chemistry. B·Achintya KunduMinhaeng Cho
Apr 14, 2019·ACS Chemical Neuroscience·Rodrigo Aguayo-Ortiz, Laura Dominguez
Apr 3, 2020·Journal of the American Chemical Society·Nadide AltincekicHarald Schwalbe
Apr 11, 2020·The Journal of Physical Chemistry. B·Tuan Nurul Azura Tuan KobMuhammad Alif Mohammad Latif
Sep 26, 2021·Proceedings of the National Academy of Sciences of the United States of America·Denis MaagTomáš Kubař

❮ Previous
Next ❯

Methods Mentioned

BETA
neutron diffraction

Software Mentioned

NumPy
Pymol
Pisces

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved