Arginine/serine-rich domains of SR proteins can function as activators of pre-mRNA splicing

Molecular Cell
Brenton R Graveley, T Maniatis

Abstract

Serine/arginine (SR)-rich splicing factors contain an RNA binding domain and an arginine/serine (RS)-rich domain required for protein-protein interactions. In addition to their roles in the basic splicing reaction, SR proteins function as components of splicing enhancer complexes. Here, we investigate the role of RS domains in splicing enhancer function. Hybrid proteins containing RS domains fused to the MS2 RNA binding protein were tested in vitro with RNA substrates bearing an MS2 recognition sequence. These hybrid proteins activated splicing in nuclear extracts, but not in S100 extracts lacking SR proteins. However, intact recombinant SR proteins could complement the activity of the hybrid proteins in S100 extracts. These data demonstrate that RS domains function as splicing activators and suggest that the general and enhancer-dependent functions of SR proteins can be uncoupled.

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M ZhangM R Green
Dec 5, 1992·Journal of Molecular Biology·D SchneiderL Gold
Apr 10, 1992·Science·M Tian, T Maniatis
May 1, 1992·Genes & Development·A M ZahlerM B Roth
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·P Zuo, J L Manley
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·A M Zahler, M B Roth
Jul 15, 1994·Genes & Development·M Tian, T Maniatis
Mar 1, 1993·Genes & Development·A WatakabeY Shimura
Jul 1, 1996·Genes & Development·J L Manley, R Tacke
Feb 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·R TackeJ L Manley
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·S D ChandlerX D Fu
Jun 1, 1997·Current Opinion in Cell Biology·K J HertelT Maniatis
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·B GranadinoL Sánchez

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Citations

Aug 1, 2000·Trends in Biochemical Sciences·C W Smith, J Valcárcel
Jun 26, 2001·RNA·B R GraveleyT Maniatis
Oct 31, 2002·RNA·Bianca J Lam, Klemens J Hertel
Jul 20, 1999·RNA·J R Sanford, J P Bruzik
Oct 6, 2009·Nature Methods·Yang WangZefeng Wang
Sep 17, 2008·Nature Structural & Molecular Biology·Ying FengJames L Manley
Sep 18, 2012·Nature Structural & Molecular Biology·Yang WangZefeng Wang
Dec 18, 2012·Nature Structural & Molecular Biology·Yang WangZefeng Wang
Mar 21, 2003·The Journal of Biological Chemistry·Bianca J LamKlemens J Hertel
Feb 3, 2011·Molecular & Cellular Proteomics : MCP·Becky Pinjou TsaiMarian L Waterman
Jan 11, 2000·Nucleic Acids Research·W van Der Houven Van OordtJ F Cáceres
Nov 7, 2003·Nucleic Acids Research·Dana PhilippsBrenton R Graveley
Jan 13, 2006·Nucleic Acids Research·Chad Haynes, Lilia M Iakoucheva
Dec 1, 2006·Nucleic Acids Research·Kathryn S Sciabica, Klemens J Hertel
Jul 1, 1999·Genes & Development·J R Sanford, J P Bruzik
Dec 22, 2000·Genes & Development·J Zhu, A R Krainer
Apr 15, 2004·Genes & Development·Jeremy R SanfordJavier F Cáceres
Jan 1, 2009·Genome Research·Jeremy R SanfordYunlong Liu
Jul 14, 2011·Genome Research·Timothy Sterne-WeilerJeremy R Sanford
Jul 30, 2008·Molecular and Cellular Biology·Anyu ZhouShu-Ching Huang
Aug 12, 2009·Molecular and Cellular Biology·Hazeem L Okunola, Adrian R Krainer
Oct 18, 2001·Molecular and Cellular Biology·S GuthJ Valcárcel
Jul 9, 2002·Molecular and Cellular Biology·Suzanne Furuyama, James P Bruzik
Sep 7, 2002·Molecular and Cellular Biology·Demian CazallaJavier F Cáceres
Jul 16, 2003·Molecular and Cellular Biology·Dawn S ChandlerWilliam Mattox
Mar 11, 2003·Annual Review of Biochemistry·Douglas L Black

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