Arginyl-tRNA synthetase from Escherichia coli K12. Purification, properties, and sequence of substrate addition

J Charlier, E Gerlo


Arginyl-tRNA synthetase from Escherichia coli K12 has been purified more than 1000-fold with a recovery of 17%. The enzyme consists of a single polypeptide chain of about 60 000 molecular weight and has only one cysteine residue which is essential for enzymatic activity. Transfer ribonucleic acid completely protects the enzyme against inactivation by p-hydroxymercuriben zoate. The enzyme catalyzes the esterification of 5000 nmol of arginine to transfer ribonucleic acid in 1 min/mg of protein at 37 degrees C and pH 7.4. One mole of ATP is consumed for each mole of arginyl-tRNA formed. The sequence of substrate binding has been investigated by using initial velocity experiments and dead-end and product inhibition studies. The kinetic patterns are consistent with a random addition of substrates with all steps in rapid equilibrium except for the interconversion of the cental quaternary complexes. The dissociation constants of the different enzyme-substrate complexes and of the complexes with the dead-end inhibitors homoarginine and 8-azido-ATP have been calculated on this basis. Binding of ATP to the enzyme is influenced by tRNA and vice versa.


Jan 1, 1984·The International Journal of Biochemistry·H Y Wang, F Pan
Jun 10, 1985·Biochimica Et Biophysica Acta·B Cirakoğlu, J P Waller
Aug 15, 1984·European Journal of Biochemistry·T MizutaniA Hashimoto
Feb 14, 2009·PLoS Computational Biology·Patrick F SuthersCostas D Maranas
Oct 6, 1980·FEBS Letters·A Joachimiak, J Barciszewski
Jan 24, 2006·Biochimica Et Biophysica Acta·R Kalervo Airas
Jun 5, 2016·International Journal of Biological Macromolecules·Shruti Bindal, Rani Gupta


May 17, 1976·European Journal of Biochemistry·J GangloffG Dirheimer
Apr 2, 1973·European Journal of Biochemistry·F von der Haar
Jul 1, 1969·Biochimica Et Biophysica Acta·R D Marshall, P C ZAMECNIK
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN
Oct 15, 1961·Proceedings of the National Academy of Sciences of the United States of America·M L STEPHENSON, P C ZAMECNIK
Jan 1, 1967·Annual Review of Biochemistry·W W Cleland

Related Concepts

Bacterial Proteins
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