Arginyl-tRNA synthetase from Escherichia coli K12. Purification, properties, and sequence of substrate addition

Biochemistry
J Charlier, E Gerlo

Abstract

Arginyl-tRNA synthetase from Escherichia coli K12 has been purified more than 1000-fold with a recovery of 17%. The enzyme consists of a single polypeptide chain of about 60 000 molecular weight and has only one cysteine residue which is essential for enzymatic activity. Transfer ribonucleic acid completely protects the enzyme against inactivation by p-hydroxymercuriben zoate. The enzyme catalyzes the esterification of 5000 nmol of arginine to transfer ribonucleic acid in 1 min/mg of protein at 37 degrees C and pH 7.4. One mole of ATP is consumed for each mole of arginyl-tRNA formed. The sequence of substrate binding has been investigated by using initial velocity experiments and dead-end and product inhibition studies. The kinetic patterns are consistent with a random addition of substrates with all steps in rapid equilibrium except for the interconversion of the cental quaternary complexes. The dissociation constants of the different enzyme-substrate complexes and of the complexes with the dead-end inhibitors homoarginine and 8-azido-ATP have been calculated on this basis. Binding of ATP to the enzyme is influenced by tRNA and vice versa.

References

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Citations

May 17, 1976·European Journal of Biochemistry·J GangloffG Dirheimer
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Related Concepts

Bacterial Proteins
Alkalescens-Dispar Group
Nevus
Talpidae
SDS-PAGE
Complex (molecular entity)
MT-TA gene
Escherichia coli K12
Triplet Codon-amino Acid Adaptor Activity
Transfer RNA

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