Arginylated calreticulin at plasma membrane increases susceptibility of cells to apoptosis.

The Journal of Biological Chemistry
Cecilia Lopez SambrooksMarta E Hallak

Abstract

Post-translational modifications of proteins are important for the regulation of cell fate and functions; one of these post-translational modifications is arginylation. We have previously established that calreticulin (CRT), an endoplasmic reticulum resident, is also one of the arginylated substrates found in the cytoplasm. In the present study, we describe that arginylated CRT (R-CRT) binds to the cell membrane and identified its role as a preapoptotic signal. We also show that cells lacking arginyl-tRNA protein transferase are less susceptible to apoptosis than wild type cells. Under these conditions R-CRT is present on the cell membrane but at early stages is differently localized in stress granules. Moreover, cells induced to undergo apoptosis by arsenite show increased R-CRT on their cell surface. Exogenously applied R-CRT binds to the cell membrane and is able to both increase the number of cells undergoing apoptosis in wild type cells and overcome apoptosis resistance in cells lacking arginyl-tRNA protein transferase that express R-CRT on the cell surface. Thus, these results demonstrate the importance of surface R-CRT in the apoptotic response of cells, implying that post-translational arginylation of CRT can regulate i...Continue Reading

References

Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·N K SinghH L Nakhasi
Sep 1, 1994·Clinical Immunology and Immunopathology·P EggletonA I Tauber
Mar 17, 1997·Biochemical and Biophysical Research Communications·Q ZhuM L Tanzer
Jun 8, 2000·The Journal of Biological Chemistry·E F CorbettM Michalak
Aug 23, 2000·The Journal of Cell Biology·K NakamuraM Michalak
Jan 10, 2001·The Journal of Cell Biology·J M HolaskaB M Paschal
Mar 30, 2002·The Journal of Biological Chemistry·Anthony Wayne OrrJoanne E Murphy-Ullrich
Jul 31, 2002·The Journal of Biological Chemistry·Silvia GoicoecheaJoanne E Murphy-Ullrich
Sep 21, 2002·Molecular and Cellular Biology·Lubov T TimchenkoNikolai A Timchenko
Apr 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Maria H HamiltonJohn D Hildebrandt
Jun 25, 2003·The Journal of Cell Biology·Anthony Wayne OrrJoanne E Murphy-Ullrich
Apr 21, 2004·Protein Science : a Publication of the Protein Society·Ann-Maree CatanzaritiRohan T Baker
Apr 9, 2005·The Journal of Biological Chemistry·Dequan ChenMarlyn P Langford
Oct 4, 2005·Molecular and Cellular Biology·Nima AfsharBryce M Paschal
Apr 26, 2006·Journal of Cell Science·Natalia DolzhanskayaRobert B Denman
Jun 24, 2006·Science·Marina KarakozovaAnna Kashina
Oct 31, 2006·The Journal of Investigative Dermatology. Symposium Proceedings·Leslie I GoldCaprice L Cadacio
Dec 26, 2006·Nature Medicine·Michel ObeidGuido Kroemer
Jan 2, 2007·The Journal of Biological Chemistry·María B DeccaMarta E Hallak
May 4, 2007·Molecular Biology of the Cell·Rachid MazrouiImed-Eddine Gallouzi
Jul 6, 2007·Autophagy·Noboru Mizushima, Tamotsu Yoshimori
Aug 8, 2007·Annual Review of Physiology·Yiping Rong, Clark W Distelhorst
Nov 6, 2007·Immunological Reviews·Michel ObeidGuido Kroemer
Feb 23, 2008·Trends in Biochemical Sciences·Paul Anderson, Nancy Kedersha
Aug 30, 2008·The American Journal of Pathology·Lillian B NanneyLeslie I Gold
Oct 7, 2008·Nature Cell Biology·Kyoko ArimotoMutsuhiro Takekawa
Jan 13, 2009·The Biochemical Journal·Marek MichalakMichal Opas
Jan 24, 2009·The EMBO Journal·Theocharis PanaretakisGuido Kroemer
Apr 17, 2009·Molecular Biology of the Cell·Sophie MokasRachid Mazroui
Nov 11, 2009·Cell Cycle·Oliver KeppGuido Kroemer
Jan 13, 2010·Molecular Cell·J Ross Buchan, Roy Parker
Apr 29, 2010·The Biochemical Journal·Marcos A CarpioMarta E Hallak
Jul 14, 2010·Journal of Molecular Biology·Joanna M TarrPaul Eggleton

❮ Previous
Next ❯

Citations

Apr 10, 2013·The International Journal of Biochemistry & Cell Biology·Marcos A CarpioMarta E Hallak
Aug 16, 2014·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Anna Kashina
Mar 10, 2016·Expert Opinion on Therapeutic Targets·Paul EggletonMarek Michalak
Jun 19, 2016·Journal of Neurochemistry·Mauricio R GalianoMarta E Hallak
Mar 3, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fang-Yuan GongXiao-Ming Gao
May 15, 2015·The Journal of Biological Chemistry·Victor E Goitea, Marta E Hallak
Jun 20, 2018·Molecular Neurobiology·Andrea CombaMauricio R Galiano
Apr 1, 2019·Apoptosis : an International Journal on Programmed Cell Death·Alejandro Schcolnik-CabreraFela Mendlovic
Jan 8, 2021·Frontiers in Cell and Developmental Biology·Chunhua JiangFangliang Zhang
Jun 20, 2020·Cell Chemical Biology·Irem Avcilar-KucukgozeAnna Kashina

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis