Argonaute2 is a potential target for siRNA-based cancer therapy for HT1080 human fibrosarcoma

Drug Delivery and Translational Research
Tatsuaki TagamiHiroshi Kiwada

Abstract

Small interfering RNAs (siRNAs) are small RNA molecules that have a potent, sequence-specific gene silencing effect and therefore show promise for therapeutic use as molecular-targeted drugs for the treatment of various genetic diseases, including cancer. The aim of the present study was to evaluate whether Argonaute2 (Ago2) is a therapeutically effective target for siRNA-based cancer therapy. Ago2 is the key protein in mammalian RNAi and is also known as the only member of the Ago family that mediates the microRNA (miRNA)-dependent cleavage of targeted mRNAs. It is assumed that these unique properties of the Ago2 protein can play a central role in the regulation of the miRNA pathway and subsequent translational inhibition of miRNA-targeted mRNAs, including cell survival and cancer progression. To assess its therapeutic effect, siRNA against Ago2 (Ago2-siRNA) was transfected into HT1080 human fibrosarcoma cells, which are malignant cancer cells. Ago2 gene silencing resulted in the inhibition of cell growth and the induction of apoptosis and G0/G1 arrest in the cell cycle. In addition, Ago2 knockdown induced morphological changes and actin stress fiber formation in the cells. The results of a microarray study showed that Ago2 su...Continue Reading

References

Feb 1, 1992·Current Opinion in Oncology·J Fawcett, A L Harris
Jan 1, 1985·The Journal of Bone and Joint Surgery. British Volume·W K Taconis, T G van Rijssel
Dec 16, 1993·Nature·Y XiongD Beach
Aug 9, 2003·Genomics·Takashi SasakiNobuyoshi Shimizu
Sep 23, 2003·Expert Opinion on Therapeutic Targets·Stella Pelengaris, Mike Khan
May 20, 2004·Cancer Research·Yoshifumi TakeiTakashi Muramatsu
Jul 21, 2004·Molecular Cell·Gunter MeisterThomas Tuschl
Jul 31, 2004·Science·Jidong LiuGregory J Hannon
Sep 17, 2004·Nature·Gunter Meister, Thomas Tuschl
Apr 20, 2005·Oncogene·Desssislava K Dimova, Nicholas J Dyson
Dec 13, 2005·Cancer Treatment Reviews·Simone MocellinDonato Nitti
Mar 7, 2006·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Cinzia SevignaniCarlo M Croce
Aug 8, 2006·Seminars in Oncology·John Mendelsohn, Jose Baselga
Sep 26, 2006·International Journal of Pharmaceutics·Tomotaka KobayashiHiroshi Kiwada
Nov 14, 2006·International Journal of Pharmaceutics·Tatsuaki TagamiHiroshi Kiwada
Nov 23, 2006·Melanoma Research·Rene GonzalezPaul O Schwarzenberger
Mar 21, 2007·Trends in Genetics : TIG·Timothy W Nilsen
Apr 6, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jie LiangDaiming Fan
Apr 6, 2007·Biological & Pharmaceutical Bulletin·Lap Thi NguyenHiroshi Kiwada
Apr 26, 2007·Applied Microbiology and Biotechnology·Achim Aigner
Jul 14, 2007·Genes & Development·Dónal O'CarrollAlexander Tarakhovsky
Sep 27, 2007·Molecular Cancer·William C S Cho
Dec 7, 2007·The Journal of Clinical Investigation·Saghir Akhtar, Ibrahim F Benter
Jan 29, 2008·Biochemical and Biophysical Research Communications·Tomohiro AsaiNaoto Oku
Apr 16, 2008·Expert Opinion on Therapeutic Targets·Chuan HuangQizhi Yao
Jul 2, 2008·Expert Reviews in Molecular Medicine·Abde M Abukhdeir, Ben Ho Park
Sep 18, 2008·Cancer Investigation·Nandita SaxenaRajendra Prasad Tripathi
Mar 17, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Amr S Abu LilaHiroshi Kiwada
Jun 30, 2009·Cancer Treatment Reviews·Bríd M RyanMichael J Duffy
Jan 14, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yan Degenhardt, Thomas Lampkin
Jan 15, 2010·Methods in Molecular Biology·José Mario BarichelloHiroshi Kiwada
Feb 6, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Talar Tokatlian, Tatiana Segura
Jun 22, 2010·Molecular Carcinogenesis·Anna G AntonacopoulouHaralabos P Kalofonos

❮ Previous
Next ❯

Citations

Nov 22, 2011·International Journal of Pharmaceutics·Tatsuaki TagamiHiroshi Kiwada
Mar 2, 2013·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Tatsuhiro Ishida, Hiroshi Kiwada

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

Cancer Metabolism: Therapeutic Targets

Targeting the mechanisms by which cancer cells acquire energy for metabolic needs is a therapeutic target. Discover the latest research on cancer metabolism and therapeutic targets.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis