ArgR-regulated genes are derepressed in the Legionella-containing vacuole.

Journal of Bacteriology
Galadriel Hovel-MinerH A Shuman

Abstract

Legionella pneumophila is an intracellular pathogen that infects protozoa in aquatic environments and when inhaled by susceptible human hosts replicates in alveolar macrophages and can result in the often fatal pneumonia called Legionnaires' disease. The ability of L. pneumophila to replicate within host cells requires the establishment of a specialized compartment that evades normal phagolysosome fusion called the Legionella-containing vacuole (LCV). Elucidation of the biochemical composition of the LCV and the identification of the regulatory signals sensed during intracellular replication are inherently challenging. L-Arginine is a critical nutrient in the metabolism of both prokaryotic and eukaryotic organisms. We showed that the L. pneumophila arginine repressor homolog, ArgR, is required for maximal intracellular growth in the unicellular host Acanthamoeba castellanii. In this study, we present evidence that the concentration of L-arginine in the LCV is sensed by ArgR to produce an intracellular transcriptional response. We characterized the L. pneumophila ArgR regulon by global gene expression analysis, identified genes highly affected by ArgR, showed that ArgR repression is dependent upon the presence of L-arginine, and...Continue Reading

References

Dec 1, 1977·The New England Journal of Medicine·D W FraserP S Brachman
Jul 1, 1979·Journal of Clinical Microbiology·W J Warren, R D Miller
Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A MarraH A Shuman
Mar 1, 1989·Seminars in Respiratory Infections·R R MuderG D Fang
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D B LimW K Maas
Dec 1, 1984·The Journal of Cell Biology·M A Horwitz, F R Maxfield
Mar 1, 1980·Journal of Clinical Microbiology·J R GeorgeW K Harrell
Apr 1, 1981·Journal of Clinical Microbiology·M W ReevesW K Harrell
Sep 1, 1980·The Journal of Clinical Investigation·M A Horwitz, S C Silverstein
Nov 24, 1995·Journal of Molecular Biology·R GrandoriJ Carey
Dec 1, 1994·Microbiological Reviews·W K Maas
Aug 1, 1994·Molecular Microbiology·G Tian, W K Maas
Jul 1, 1996·Trends in Microbiology·B S Fields
Jan 1, 1997·Annual Review of Microbiology·A P Sinai, K A Joiner
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·G SegalH A Shuman
Aug 26, 1998·Trends in Microbiology·G Segal, H A Shuman
May 20, 1999·Nature Structural Biology·J NiG D Van Duyne
Oct 6, 2000·Annual Review of Microbiology·M S Swanson, B K Hammer
Oct 3, 2001·Journal of Molecular Biology·D SzwajkajzerJ Carey
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 23, 2002·Molecular Microbiology·Brian K HammerMichele S Swanson
May 11, 2002·Journal of Bacteriology·Alexandros K Kiupakis, Larry Reitzer
Jun 19, 2002·FEMS Microbiology Reviews·Michael SteinertJörg Hacker
Oct 18, 2002·Nucleic Acids Research·Adel M TalaatStephen Albert Johnston
Nov 26, 2002·Nature Cell Biology·Jonathan C Kagan, Craig R Roy

❮ Previous
Next ❯

Citations

Apr 18, 2013·PLoS Genetics·Kirsten Maren EllegaardSiv G E Andersson
Jan 8, 2015·Frontiers in Microbiology·Peter RobertsonRafael A Garduño
Sep 25, 2014·Frontiers in Cellular and Infection Microbiology·Christian Manske, Hubert Hilbi
Jul 28, 2016·FEBS Letters·Wolfgang Eisenreich, Klaus Heuner
Aug 26, 2016·BMC Microbiology·Bronwyn G ButcherMelanie J Filiatrault
Mar 13, 2016·Applied and Environmental Microbiology·Kelsi M SandozRobert A Heinzen
May 18, 2016·Infection and Immunity·Tamara J O'ConnorRalph R Isberg
Jun 12, 2016·Applied and Environmental Microbiology·Christian ManskeHubert Hilbi
Dec 24, 2018·The Journal of Biological Chemistry·Nadezhda LevanovaGunnar Neels Schroeder
Feb 13, 2019·Journal of Bacteriology·Derek E MoormeierRobert A Heinzen
Aug 23, 2019·Proceedings of the National Academy of Sciences of the United States of America·Eric T ChristensonAnirban Banerjee
May 4, 2017·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Abdul Qader Tahir Ismail

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.