ArHsp40 and ArHsp40-2 contribute to stress tolerance and longevity in Artemia franciscana , but only ArHsp40 influences diapause entry

The Journal of Experimental Biology
Nathan M Rowarth, Thomas H MacRae

Abstract

Embryos of the crustacean Artemia franciscana develop either ovoviviparously or oviparously, yielding swimming larvae (nauplii) or encysted gastrulae (cysts), respectively. Nauplii moult several times and become adults whereas cysts enter diapause, a state of dormancy characterized by exceptionally low metabolism and high stress tolerance. Synthesis of molecular chaperones such as the J-domain proteins ArHsp40 and ArHsp40-2 occurs during embryo development and post-diapause growth of A. franciscana and they influence development and stress tolerance. To further investigate J-domain protein function, ArHsp40 and ArHsp40-2 were each knocked down by RNA interference. Reductions in ArHsp40 and ArHsp40-2 had no effect on adult survival, time to release of cysts and nauplii from females and first-brood size. However, knockdown of both A. franciscana J-domain proteins reduced the longevity and heat tolerance of nauplii, with the loss of ArHsp40 having a greater effect. The knockdown of ArHsp40, but not of ArHsp40-2, caused approximately 50% of cysts to abort diapause entry and hatch without exposure to an exogenous signal such as low temperature and/or desiccation. Cysts lacking ArHsp40 that entered diapause exhibited decreased stress...Continue Reading

References

Sep 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·H Xiang, T H MacRae
Jul 25, 1997·The Journal of Biological Chemistry·P LiangT H MacRae
Dec 1, 2001·Annual Review of Entomology·David L Denlinger
Feb 28, 2004·Seminars in Cell & Developmental Biology·Thomas H MacRae
Mar 17, 2005·Cellular and Molecular Life Sciences : CMLS·M P Mayer, B Bukau
Dec 8, 2005·Journal of Insect Physiology·Vladimír Kostál
May 29, 2007·Journal of Insect Physiology·Daniel A Hahn, David L Denlinger
Mar 10, 2010·Cellular and Molecular Life Sciences : CMLS·Thomas H MacRae
Apr 20, 2010·The Journal of Experimental Biology·Heather M RobbinsPeter Bossier
Nov 1, 2005·Integrative and Comparative Biology·James S Clegg
Jan 19, 2012·PloS One·Melody S ClarkEsther Lubzens
Aug 13, 2013·Insect Biochemistry and Molecular Biology·Julie A ReynoldsDavid L Denlinger
Oct 29, 2013·Annual Review of Entomology·David L Denlinger, Peter A Armbruster
Oct 24, 2014·Annual Review of Entomology·Allison M King, Thomas H MacRae
Dec 10, 2014·Sub-cellular Biochemistry·Douglas M Cyr, Carlos H Ramos
Aug 8, 2015·Nature·Nadinath B NillegodaBernd Bukau
Sep 4, 2015·Cell Stress & Chaperones·Thomas H MacRae
Feb 22, 2017·The EMBO Journal·Szymon ŻwirowskiKrzysztof Liberek
Feb 22, 2017·Cell Stress & Chaperones·Erik R P ZuiderwegJason E Gestwicki
Mar 16, 2017·Molecular and Cellular Biology·Jayasankar Mohanakrishnan KaimalClaes Andréasson
Mar 17, 2017·Insect Biochemistry and Molecular Biology·Julie A ReynoldsDavid L Denlinger
Mar 21, 2017·Trends in Biochemical Sciences·Elizabeth A Craig, Jaroslaw Marszalek
Jul 8, 2017·Protein Science : a Publication of the Protein Society·Neil Andrew D BascosSamuel J Landry
Jul 20, 2017·Proceedings of the National Academy of Sciences of the United States of America·Vladimír KoštálAlexander William Bruce

❮ Previous
Next ❯

Citations

Nov 7, 2019·Journal of Experimental Botany·Nathan M RowarthArunika Hlan Gunawardena
Aug 15, 2020·Cell Stress & Chaperones·Yayra A GbotsyoThomas H MacRae
Nov 17, 2020·Frontiers in Physiology·Jonathan D HibshmanBob Goldstein
Sep 29, 2020·Developmental and Comparative Immunology·Wisarut JunprungAnchalee Tassanakajon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.