ARL2 overexpression inhibits glioma proliferation and tumorigenicity via down-regulating AXL

BMC Cancer
Yulin WangZongze Guo

Abstract

Glioma is the most common primary brain tumor in adults with a poor prognosis. As a member of ARF subfamily GTPase, ARL2 plays a key role in regulating the dynamics of microtubules and mitochondrial functions. Recently, ARL2 has been identified as a prognostic and therapeutic target in a variety range of malignant tumors. However, the biological functional role of ARL2 in glioma still remains unknown. The aim of this study was to explore the expression and functional role of ARL2 in glioma. In this study, we investigated the expression of ARL2 in glioma samples by using RT-PCR, immunohistochemistry and western blot. The correlation between ARL2 expression and the outcomes of glioma patients was evaluated with survival data from TCGA, CGGA and Rembrandt dataset. Lentiviral technique was used for ARL2 overexpression in U87 and U251 cells. CCK8 assay, colony formation assay, wound healing test, transwell invasion assay and in vivo subcutaneous xenograft model were performed to investigated the biological functions of ARL2. ARL2 expression was down-regulated in glioma, and was inversely associated with poor prognosis in glioma patients. Furthermore, exogenous ARL2 overexpression attenuated the growth and colony-formation abilities ...Continue Reading

References

Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J ClarkR A Kahn
Oct 12, 2000·Cellular Signalling·T MatozakiY Takai
Oct 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aravind SubramanianJill P Mesirov
Mar 10, 2006·Molecular Biology of the Cell·Chengjing ZhouRichard A Kahn
Apr 6, 2006·Proceedings of the National Academy of Sciences of the United States of America·Peter VajkoczyAxel Ullrich
Dec 26, 2006·Experimental Cell Research·Anne BeghinCharles Dumontet
Feb 1, 2008·International Immunology·Ryuta MuromotoTadashi Matsuda
Aug 2, 2008·The New England Journal of Medicine·Patrick Y Wen, Santosh Kesari
Nov 5, 2008·The Journal of Biological Chemistry·Laura K BaileyHelen R Mott
Oct 18, 2011·Nature Chemical Biology·Shehab A IsmailAlfred Wittinghofer
Jul 4, 2012·Nature Genetics·Zhenfeng ZhangTrever G Bivona
Oct 1, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrea M GriesingerNicholas K Foreman
Mar 22, 2014·Cancer Cell·Andrew G StephenFrank McCormick
Oct 18, 2014·Nature Reviews. Drug Discovery·Adrienne D CoxChanning J Der
Feb 12, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Howard A Fine
Feb 20, 2016·Cellular and Molecular Life Sciences : CMLS·Fuquan Zhang, Jit Kong Cheong
Mar 10, 2016·The Journal of Cell Biology·Keng ChenHongyan Wang
Mar 12, 2016·Cancer Letters·Tao JiangUNKNOWN Chinese Glioma Cooperative Group (CGCG)
Feb 1, 2017·Biochemical and Biophysical Research Communications·Ruiqing PengJing Ren
May 19, 2017·The American Journal of Chinese Medicine·Wen-Shin WuWen-Ta Chiu
Aug 30, 2017·The EMBO Journal·Atsuko Minowa-NozawaIchiro Nakagawa

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Citations

Jun 27, 2019·Journal of Translational Medicine·Yulin WangMinghua Zhuang
May 15, 2020·Bioscience Reports·Delong ZhangHui Yang
May 20, 2020·Frontiers in Cell and Developmental Biology·Cristina CasalouDuarte C Barral
Dec 4, 2020·Journal of Cellular and Molecular Medicine·Virginie VandewalleViolaine Havelange
Jun 11, 2021·Cellular Signalling·Changchun CaoLianshu Ding

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Methods Mentioned

BETA
surgical resection
GTPase
PCR
xenograft
ubiquitination

Software Mentioned

Gene Set Enrichment Analysis ( GSEA )
GlioVis
Gene Set Enrichment analysis ( GSEA
GSEA
Image J
STRING
Ubibrowser
Excel
Graphpad Prism

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