PMID: 3747512Jul 1, 1986Paper

Aromatase activity in human skin fibroblasts: characterization by an enzymatic method

Journal of Steroid Biochemistry
A ChababC Sultan

Abstract

Human skin fibroblasts were incubated for 24 h with 10(-6) M androstenedione and the estrone + estradiol released in the culture medium were measured by an enzymatic assay. Aromatase activity was expressed as pmol (estrone + estradiol) formed in the medium per mg cell protein per day. Using this method we were able to investigate the kinetic properties of aromatase in different cell strains and its stimulation by dexamethasone. Values of 92 nM and 9.1 pmol/mg protein/day were obtained respectively for Km and Vmax in cultured fibroblasts derived from genital skin of normal prepubertal subjects. In patients with complete androgen insensitivity syndrome CAIS, the Km was 156 nM and the Vmax 42 pmol/mg protein/day. Aromatase activity varied from 7.9 +/- 1.2 pmol/mg protein/day (mean +/- SD; n = 19) in normal prepubertal boys to 24.5 +/- 4.7 pmol/mg protein/day (mean +/- SD; n = 11) in those from normal postpubertal boys. The values were even higher in fibroblasts cultured from genital skin of prepubertal patients with CAIS. Cell concentrations did not modify the pattern of estrogen formation and aromatase activity did not vary with serial subcultures. The stimulatory effect of dexamethasone on aromatase activity in cultured fibrobla...Continue Reading

References

Feb 15, 1979·Clinica Chimica Acta; International Journal of Clinical Chemistry·J C NicolasA Crastes de Paulet
Jul 1, 1979·Acta Endocrinologica·H K KleyH L Krüskemper
Oct 1, 1976·The Journal of Clinical Endocrinology and Metabolism·H U SchweikertJ D Wilson
Mar 1, 1974·The Journal of Clinical Endocrinology and Metabolism·D L HemsellP C MacDonald
Oct 1, 1984·The Journal of Clinical Endocrinology and Metabolism·G D BerkovitzC J Migeon
Nov 1, 1983·Analytical Biochemistry·J C NicolasA Crastes de Paulet
Mar 1, 1984·The Journal of Clinical Endocrinology and Metabolism·E PerelD W Killinger
Feb 1, 1984·Journal of Steroid Biochemistry·E J Folkerd, V H James
Jul 1, 1983·Journal of Steroid Biochemistry·E J Folkerd, V H James
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·E R SimpsonC R Mendelson
Apr 13, 1981·Molecular and Cellular Biochemistry·T R Brown, C J Migeon
Aug 1, 1981·The Journal of Clinical Endocrinology and Metabolism·G E AckermanE R Simpson
Jan 1, 1980·Molecular and Cellular Endocrinology·I MowszowiczP Mauvais-Jarvis
Jan 1, 1980·Pediatric Research·C SultanC J Migeon

❮ Previous
Next ❯

Citations

Jul 1, 1986·Journal of Steroid Biochemistry·A ChababB Descomps
Mar 1, 1992·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·M A BlankensteinJ H Thijssen
Dec 11, 2002·Experimental Dermatology·M J Thornton

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.