Aromatase knockout mice show normal steroid-induced activation of gonadotrophin-releasing hormone neurones and luteinising hormone surges with a reduced population of kisspeptin neurones in the rostral hypothalamus

Journal of Neuroendocrinology
L Szymanski, Julie Bakker

Abstract

We recently reported that female aromatase knockout (ArKO) mice show deficits in sexual behaviour and a decreased population of kisspeptin-immunoreactive neurones in the rostral periventricular area of the third ventricle (RP3V), resurrecting the question of whether oestradiol actively contributes to female-typical sexual differentiation. To further address this question, we assessed the capacity of ArKO mice to generate a steroid-induced luteinising hormone (LH) surge. Adult, gonadectomised wild-type (WT) and ArKO mice were given silastic oestradiol implants s.c. and, 1 week later, received s.c. injections of either oestradiol benzoate (EB) followed by progesterone, EB alone, or no additional steroids to activate gonadotrophin-releasing hormone (GnRH) neurones and generate an LH surge. Treatment with EB and progesterone induced significant Fos/GnRH double-labelling and, consequently, an LH surge in female WT and in ArKO mice of both sexes but not in male WT mice. ArKO mice of both sexes had fewer cells expressing Kiss-1 mRNA in the RP3V compared to female WT mice but had more Kiss-1 mRNA-expressing cells compared to WT males, reflecting an incomplete sexual differentiation of this system. To determine the number of cells expre...Continue Reading

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Citations

Mar 19, 2013·Frontiers in Neuroendocrinology·Jenny Clarkson
Jul 20, 2014·Biochimica Et Biophysica Acta·Pascal CoumailleauOlivier Kah
Dec 14, 2019·The European Journal of Neuroscience·Badr KhbouzCharlotte A Cornil
Dec 13, 2017·Proceedings of the National Academy of Sciences of the United States of America·Brian P KenealyEi Terasawa
Oct 31, 2018·Frontiers in Molecular Neuroscience·Dusti A ShayCheryl S Rosenfeld
Mar 25, 2020·International Journal of Molecular Sciences·Ariel L NegrónMaricedes Acosta-Martínez

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