PMID: 3320558Jan 1, 1987Paper

Aromatase

Journal of Steroid Biochemistry
Y OsawaC Yarborough

Abstract

Aromatase catalyzes the conversion of androgens to estrogens through a series of monooxygenations to achieve the 19-desmolation and aromatization of the neutral steroid ring-A structure. We have separated two forms of aromatase, a major (P2a) and a minor (P3) form, from human term placenta through solubilization and chromatography. Partially purified aromatase in each form was immunoaffinity chromatographed to give a single band (SDS-PAGE) cytochrome P-450 of 55 kDa, utilizing a mouse monoclonal anti-human placental aromatase cytochrome P-450 IgGi (MAb3-2C2) which is capable of suppressing placental aromatase activity. The purified cytochrome P-450 showed specific aromatase activity of 25-30 nmol/min per mg with Km of 20-30 nM for androstenedione on reconstitution with NADPH-cyt P-450 reductase and dilauroyl L-alpha-phosphatidylcholine. This one step represents a higher than 100-fold purification with maintenance of the same Km. The stability analysis showed a half-life of more than 5 yr for solubilized aromatase and 2 months for the aromatase cytochrome P-450 on storage at -90 degrees C. Contrary to the recent claim that estrogen biosynthesis by reconstituted human placental cytochrome P-450 is by trans-diaxial 1 alpha,2 beta-...Continue Reading

References

Apr 29, 1986·Biochemical and Biophysical Research Communications·N Muto, L Tan
Jan 29, 1986·Biochemical and Biophysical Research Communications·S NakajinP F Hall
May 1, 1974·The Journal of Clinical Endocrinology and Metabolism·D G Spaeth, Y Osawa
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Citations

Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J L Gaillard
Jul 1, 1994·Domestic Animal Endocrinology·K M EisenhauerJ F Roser
Dec 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·N KadohamaY Osawa
Dec 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·I Hanukoglu
Mar 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·K R KorzekwaY Osawa
Jan 7, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·S MoslemiJ L Gaillard
Jun 1, 1990·Baillière's Clinical Endocrinology and Metabolism·A J Conley, J I Mason
Oct 8, 2009·The Journal of Steroid Biochemistry and Molecular Biology·Debashis GhoshWalter Pangborn
Apr 1, 1997·The Journal of Steroid Biochemistry and Molecular Biology·Y OsawaC Yarborough
Jan 28, 2010·Expert Opinion on Drug Metabolism & Toxicology·Martin SvobodaTheresia Thalhammer
Nov 30, 1988·Biochemical and Biophysical Research Communications·D F CoveyM Cabell
Aug 20, 2019·Basic & Clinical Pharmacology & Toxicology·Robert KrysiakBogusław Okopień
May 1, 2021·International Journal of Molecular Sciences·Tushar Singh BarwalAklank Jain
May 13, 1993·Biochimica Et Biophysica Acta·N KadohamaY Osawa

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