Aromatase/Seladin-1 Interactions in Human Neuronal Cell Culture, the Hippocampus of Healthy Rats and Transgenic Alzheimer's Disease Mice

Pharmacology
Hande KarahanPelin Kelicen-Uğur

Abstract

Decreasing levels of aromatase and seladin-1 could be one of the molecular mechanisms of Alzheimer's disease (AD). Aromatase is an enzyme that catalyzes estrogen biosynthesis from androgen precursors, and seladin-1 is an enzyme that converts desmosterol to cholesterol, which is the precursor of all hormones. Verifying the potential relationship between these proteins and accordingly determining new therapeutic targets constitute the aims of this study. Changes in protein levels were compared in vitro in aromatase and seladin-1 inhibitor-administered human neuroblastoma (SH-SY5Y) cells in vivo in intracerebroventricular (icv) aromatase or seladin-1 inhibitor-administered rats, as well as in transgenic AD mice in which the genes encoding these proteins were knocked out. In the cell cultures, we observed that seladin-1 protein levels increased after aromatase enzyme inhibition. The hippocampal aromatase protein levels decreased following chronic seladin-1 inhibition in icv inhibitor-administered rats; however, the aromatase levels in the dentate gyrus of seladin-1 knockout (SelKO) AD male mice increased. These findings indicate a partial relationship between these proteins and their roles in AD pathology.

References

Apr 1, 1977·Endocrinology·G V CallardK J Ryan
Oct 1, 1991·Trends in Pharmacological Sciences·J Hardy, D Allsop
Mar 20, 2001·Archives of Neurology·S SeshadriD A Drachman
Mar 29, 2001·Physiological Reviews·D J Selkoe
May 15, 2001·Journal of Neurobiology·I AzcoitiaL M Garcia-Segura
Apr 18, 2003·Hippocampus·Janine Prange-KielGabriele M Rune
Mar 3, 2004·Annals of the New York Academy of Sciences·Inigo AzcoitiaLuis M Garcia-Segura
Dec 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Xu YueRena Li
Jun 13, 2006·Cellular and Molecular Neurobiology·Pelin Kelicen, Agneta Nordberg
Sep 5, 2008·Journal of Molecular Endocrinology·Alessandro Peri, Mario Serio
Nov 26, 2009·Biochimica Et Biophysica Acta·James W SimpkinsJames A Dykens
Nov 26, 2009·The Journal of Steroid Biochemistry and Molecular Biology·Laura H CokerSally A Shumaker
May 6, 2010·Journal of Alzheimer's Disease : JAD·Jose Viña, Ana Lloret
Jun 28, 2011·Neuroscience·R C MelcangiL M Garcia-Segura
Jun 4, 2013·The Journal of Steroid Biochemistry and Molecular Biology·Victor W Henderson
Jan 15, 2014·Molecular and Cellular Endocrinology·Rena LiYong Shen
Nov 27, 2014·Nature Reviews. Neuroscience·Maria-Angeles ArevaloLuis M Garcia-Segura
Jan 9, 2015·Molecular Neurodegeneration·Katherine R SadleirRobert Vassar
Jun 4, 2015·Seminars in Reproductive Medicine·Serdar E BulunMatthew Dyson
Jun 19, 2015·Journal of Endocrinological Investigation·A Peri
Jun 23, 2015·F1000prime Reports·Shireen SindiMiia Kivipelto
May 12, 2016·Journal of Assisted Reproduction and Genetics·Elham HosseiniReza Aflatoonian

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Citations

Nov 19, 2019·Translational Neuroscience·Pelin Kelicen-UgurHande Karahan

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