PMID: 9529382May 16, 1998Paper

Arp2/3 complex from Acanthamoeba binds profilin and cross-links actin filaments

Molecular Biology of the Cell
R D MullinsT D Pollard

Abstract

The Arp2/3 complex was first purified from Acanthamoeba castellanii by profilin affinity chromatography. The mechanism of interaction with profilin was unknown but was hypothesized to be mediated by either Arp2 or Arp3. Here we show that the Arp2 subunit of the complex can be chemically cross-linked to the actin-binding site of profilin. By analytical ultracentrifugation, rhodamine-labeled profilin binds Arp2/3 complex with a Kd of 7 microM, an affinity intermediate between the low affinity of profilin for barbed ends of actin filaments and its high affinity for actin monomers. These data suggest the barbed end of Arp2 is exposed, but Arp2 and Arp3 are not packed together in the complex exactly like two actin monomers in a filament. Arp2/3 complex also cross-links actin filaments into small bundles and isotropic networks, which are mechanically stiffer than solutions of actin filaments alone. Arp2/3 complex is concentrated at the leading edge of motile Acanthamoeba, and its localization is distinct from that of alpha-actinin, another filament cross-linking protein. Based on localization and actin filament nucleation and cross-linking activities, we propose a role for Arp2/3 in determining the structure of the actin filament net...Continue Reading

References

Jan 1, 1989·Cell Motility and the Cytoskeleton·D A KaiserT D Pollard
Jan 1, 1986·Cell Motility and the Cytoskeleton·T D PollardM Sato
Jan 1, 1986·The Journal of Cell Biology·D A KaiserT D Pollard
Jan 29, 1985·Biochemistry·J A Cooper, T D Pollard
Jan 1, 1984·The Journal of Cell Biology·P C TsengT D Pollard
Sep 16, 1980·Biochemical and Biophysical Research Communications·S MacLean-Fletcher, T D Pollard
Aug 1, 1981·The Journal of Cell Biology·L G TilneyD J DeRosier
Nov 1, 1981·The Journal of Cell Biology·U AebiP R Smith
Jun 1, 1995·The Journal of Cell Biology·J V SmallK Anderson
Jul 19, 1994·Biochemistry·I PerelroizenM F Carlier
Feb 1, 1996·Current Opinion in Cell Biology·S Frankel, M S Mooseker
Jan 27, 1997·The Journal of Cell Biology·R D MullinsT D Pollard
Mar 1, 1997·Current Biology : CB·L M Machesky
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·R D MullinsT D Pollard
Jun 1, 1996·Trends in Cell Biology·R D MullinsT D Pollard

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Citations

Nov 13, 2003·Current Opinion in Plant Biology·Michael J Deeks, Patrick J Hussey
Feb 27, 1999·Current Opinion in Cell Biology·L M Machesky, K L Gould
Dec 31, 2002·Current Opinion in Structural Biology·Thomas D Pollard, Christopher C Beltzner
May 14, 1999·Current Opinion in Structural Biology·R D Mullins, T D Pollard
Mar 6, 1999·Current Opinion in Microbiology·S J Palmieri, B K Haarer
Nov 13, 1999·Nature Cell Biology·O D WeinerH R Bourne
Jul 3, 2003·The Plant Cell·Jaideep MathurMartin Hülskamp
Dec 1, 2001·Microbiology and Molecular Biology Reviews : MMBR·M B Goldberg
Nov 10, 2001·Annual Review of Biomedical Engineering·G W Schmid-Schönbein
Dec 28, 1999·Annual Review of Cell and Developmental Biology·D A Schafer, T A Schroer
Dec 11, 2008·BMC Cell Biology·Simon A JohnstonLaura M Machesky
Nov 25, 2011·Journal of Cell Science·Monica CrokeSteven L Teitelbaum
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·S M Ahern-DjamaliF M Hoffmann
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·R D MullinsT D Pollard
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·B BoettnerL Van Aelst
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·L M MacheskyT D Pollard
Sep 7, 2000·The Journal of Cell Biology·C YangS H Zigmond
Jan 15, 2015·Developmental Cell·Jessica L Henty-Ridilla, Bruce L Goode
Mar 26, 2013·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Yoshie NagaiEijiro Jimi
Oct 13, 2005·Molecular and Biochemical Parasitology·Ana Virel, Lars Backman
Sep 30, 2014·Wiley Interdisciplinary Reviews. RNA·Guoning LiaoGang Liu
May 8, 2015·Cellular and Molecular Life Sciences : CMLS·Marie-France CarlierUNKNOWN Cytoskeleton Dynamics and Motility group
Apr 8, 2015·International Journal for Parasitology·C M GuptaA A Sahasrabuddhe
Jun 20, 2003·Current Biology : CB·Avital A RodalDavid G Drubin
Sep 19, 1998·Current Biology : CB·S H Zigmond
Jul 27, 2002·Annual Review of Cell and Developmental Biology·Matthew D Welch, R Dyche Mullins
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·T P StosselS S Shapiro
Jan 13, 2018·Journal of Cell Science·Kristen SkruberEric A Vitriol
Jan 12, 2002·The Journal of Biological Chemistry·Fumihiko NakamuraThomas P Stossel
Jun 27, 2000·The Journal of Biological Chemistry·H MimuroC Sasakawa
Nov 7, 1999·The Journal of Biological Chemistry·H N Higgs, T D Pollard

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