Arp2/3 complex-mediated actin polymerisation occurs on specific pre-existing networks in cells and requires spatial restriction to sustain functional lamellipod extension.

Cell Motility and the Cytoskeleton
D ShaoMaryse Bailly

Abstract

The classical Arp2/3-mediated dendritic network defines the cytoskeleton at the leading edge of crawling cells, and it is generally assumed that Arp2/3-mediated actin polymerization generates the force necessary to extend lamellipods. Our previous work suggested that successful lamellipod extension required not only free barbed ends for actin polymerization but also a proper ultrastructural organization of the cytoskeleton. To further explore the structural role of the Arp2/3 complex-mediated networks in lamellipod morphology and function, we performed a detailed analysis of the ultrastructure of the Arp2/3-mediated networks, using the WA domains of Scar and WASp to generate mislocalised Arp2/3 networks in vivo, and to reconstruct de novo Arp2/3-mediated actin nucleation and polymerization on extracted cytoskeletons. We present here evidence that spatially unrestricted Arp2/3-mediated networks are intrinsically three-dimensional and multilayered by nature and, as such, cannot sustain significant polarized extension. Furthermore, such networks polymerize only at preferred locations in extracted cells, corresponding to pre-existing Arp2/3 networks, suggesting that the specific molecular organization of the actin cytoskeleton, in ...Continue Reading

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Citations

Aug 13, 2011·European Biophysics Journal : EBJ·Kinneret Keren
Sep 18, 2012·Molecular Neurobiology·Bence Rácz, Richard J Weinberg
Mar 17, 2009·Nature Cell Biology·J Bradley ZucheroR Dyche Mullins
Jun 20, 2013·Nature Reviews. Molecular Cell Biology·Jose Javier Bravo-CorderoJohn Condeelis
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Nov 13, 2009·Current Biology : CB·Alex Mogilner, Kinneret Keren
Mar 17, 2009·Cell Host & Microbe·Virginie GonzalezIsabelle Tardieux

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