PMID: 8587125Dec 1, 1995Paper

Arrangement and nucleotide sequence of the gene (fus) encoding elongation factor G (EF-G) from the hyperthermophilic bacterium Aquifex pyrophilus: phylogenetic depth of hyperthermophilic bacteria inferred from analysis of the EF-G/fus sequences

Journal of Molecular Evolution
M BocchettaP Cammarano

Abstract

The gene fus (for EF-G) of the hyperthermophilic bacterium Aquifex pyrophilus was cloned and sequenced. Unlike the other bacteria, which display the streptomycin-operon arrangement of EF genes (5'-rps12-rps7-fus-tuf-3'), the Aquifex fus gene (700 codons) is not preceded by the two small ribosomal subunit genes although it is still followed by a tuf gene (for EF-Tu). The opposite strand upstream from the EF-G coding locus revealed an open reading frame (ORF) encoding a polypeptide having 52.5% identity with an E. coli protein (the pdxJ gene product) involved in pyridoxine condensation. The Aquifex EF-G was aligned with available homologs representative of Deinococci, high G+C Gram positives, Proteobacteria, cyanobacteria, and several Archaea. Outgroup-rooted phylogenies were constructed from both the amino acid and the DNA sequences using first and second codon positions in the alignments except sites containing synonymous changes. Both datasets and alternative tree-making methods gave a consistent topology, with Aquifex and Thermotoga maritima (a hyperthermophile) as the first and the second deepest offshoots, respectively. However, the robustness of the inferred phylogenies is not impressive. The branching of Aquifex more deep...Continue Reading

Citations

Nov 28, 2013·Genome Biology and Evolution·Robert J M EveleighRobert G Beiko
Feb 13, 2001·Nucleic Acids Research·R PaulS Altman
Mar 3, 2005·Genome Research·Eric J DeedsEugene I Shakhnovich
Mar 27, 2002·Journal of Bacteriology·Arvind K Bansal, Terrance E Meyer
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Ying XuNicolas Glansdorff
Jul 31, 2012·International Journal of Evolutionary Biology·Kenro OshimaMasaharu Ishii
Sep 29, 2006·RNA·Michal MarszalkowskiDagmar K Willkomm
Jan 10, 2013·PLoS Computational Biology·Xochitl C Morgan, Curtis Huttenhower
Jan 23, 2008·Biological Chemistry·Michal MarszalkowskiRoland K Hartmann
Jan 10, 2002·Biochimica Et Biophysica Acta·J Castresana
Dec 31, 1997·Microbiology and Molecular Biology Reviews : MMBR·J R Brown, W F Doolittle

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