PMID: 7031657Nov 1, 1981Paper

Arrangement of the subunits in the ribosome of Escherichia coli: demonstration by immunoelectron microscopy

Proceedings of the National Academy of Sciences of the United States of America
B KastnerG Stöffler

Abstract

The three-dimensional locations of Escherichia coli ribosomal proteins S13, L1, and L7/L12 on the surface of ribosomal subunits and 70S monomeric ribosomes were determined by electron microscopy of antibody-labeled ribosomal particles. A new approach to orient the subunits within 70S ribosomes was developed that used 30S.70S.50S triples that were prepared by simultaneous combination with one antibody directed against a 30S protein and another directed against a 50S protein. Electron microscope studies of triples obtained with the antibody combinations anti-S13/anti-L1 and anti-S13/anti-L7/L12 showed that, in 70S monomeric ribosomes, the head of the 30S subunit is proximate to protein L1 and the peptidyl transferase center but far from the rod-like appendage containing proteins L7 and L12.

References

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Mar 25, 1979·Journal of Molecular Biology·K R Leonard, J A Lake
Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·G W TischendorfG Stöffler
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Jul 15, 1981·Journal of Molecular Biology·E R DabbsG Stöffler

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Citations

Apr 19, 1990·Biochimica Et Biophysica Acta·G Harauz, D Flannigan
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R LührmannG Stöffler
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M Stöffler-MeilickeG Stöffler
Jan 2, 1984·European Journal of Biochemistry·J A MaassenW Möller
Jan 1, 1982·Progress in Biophysics and Molecular Biology·A Liljas
Dec 25, 1983·Journal of Molecular Biology·V D VasilievS N Ryazantsev
Jun 19, 1984·Biochemistry·J S HutchisonK Moldave
Jun 5, 1987·Journal of Molecular Biology·M Leijonmarck, A Liljas
Jun 13, 1983·FEBS Letters·A S Spirin
Sep 22, 1982·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·H G Wittmann
Sep 25, 1999·Science·J H CateH F Noller

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