Array assessment of phage-displayed peptide mimics of Human Immunodeficiency Virus type 1 gp41 immunodominant epitope: binding to antibodies of infected individuals

Proteomics
Marie-Claire ArnaudVehary Sakanyan

Abstract

An array of phage-displayed mimetic peptides representing 18 sequences from the HIV-1 gp41 immunodominant epitope was fabricated on a nitrocellulose membrane. It used to compare the antibody-binding affinity of the peptide and to monitor the immune response of four patients prior to and after the initiation of Highly Active Anti-Retroviral Therapy in parallel assays with several probes using near-infrared fluorescence detection. The proposed multiplexed approach is highly sensitive, consumes less sample and can be used to analyze the immune repertoires of virus-infected individuals.

Citations

Aug 16, 2005·Expert Review of Proteomics·Oliver K BernhardAnthony L Cunningham
Nov 26, 2005·Expert Review of Proteomics·Dawn MattoonBarry Schweitzer
Mar 1, 2008·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J-C GaudinT Haertlé
Jan 1, 2008·Biomarker Insights·Mamoun Ahram, Emanuel F Petricoin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.