Array comparative genomic hybridization analysis of Trichoderma reesei strains with enhanced cellulase production properties.

BMC Genomics
Marika VitikainenMarkku Saloheimo

Abstract

Trichoderma reesei is the main industrial producer of cellulases and hemicellulases that are used to depolymerize biomass in a variety of biotechnical applications. Many of the production strains currently in use have been generated by classical mutagenesis. In this study we characterized genomic alterations in high-producing mutants of T. reesei by high-resolution array comparative genomic hybridization (aCGH). Our aim was to obtain genome-wide information which could be utilized for better understanding of the mechanisms underlying efficient cellulase production, and would enable targeted genetic engineering for improved production of proteins in general. We carried out an aCGH analysis of four high-producing strains (QM9123, QM9414, NG14 and Rut-C30) using the natural isolate QM6a as a reference. In QM9123 and QM9414 we detected a total of 44 previously undocumented mutation sites including deletions, chromosomal translocation breakpoints and single nucleotide mutations. In NG14 and Rut-C30 we detected 126 mutations of which 17 were new mutations not documented previously. Among these new mutations are the first chromosomal translocation breakpoints identified in NG14 and Rut-C30. We studied the effects of two deletions iden...Continue Reading

References

Jan 1, 1991·Journal of Biotechnology·J M UusitaloM E Penttilä
Jan 2, 1992·Gene·T W ChristiansonP Hieter
Feb 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·P A Garrity, B J Wold
Dec 31, 1997·Genes, Chromosomes & Cancer·S Solinas-ToldoP Lichter
May 26, 1999·Critical Reviews in Microbiology·F Fierro, J F Martín
Sep 2, 1999·Nature Genetics·J R PollackP O Brown
Feb 26, 2000·Molecular Biology of the Cell·H Jin, D C Amberg
Nov 15, 2001·Cellular and Molecular Life Sciences : CMLS·R C May
Feb 26, 2004·Nature Genetics·Adrian S IshkanianWan L Lam
Oct 6, 2004·Genome Biology·Robert C GentlemanJianhua Zhang
Dec 24, 2005·Nature·Masayuki MachidaHisashi Kikuchi
May 2, 2006·Statistical Applications in Genetics and Molecular Biology·Gordon K Smyth
Jun 9, 2006·Cell Cycle·Hiroki KurahashiBeverly S Emanuel
Jun 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Hildur V ColotJay C Dunlap
Sep 12, 2006·Nucleic Acids Research·Cathryn RehmeyerMark Farman
Sep 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Kazuma IshibashiHirokazu Inoue
Jan 20, 2007·Bioinformatics·E S Venkatraman, Adam B Olshen
Jun 28, 2007·BMC Bioinformatics·Joern ToedlingWolfgang Huber
Feb 12, 2008·Current Protocols in Molecular Biology·P R MuellerP A Garrity
Apr 12, 2008·PLoS Genetics·Natalie D FedorovaWilliam C Nierman
Aug 12, 2008·Bioinformatics·Erez Ben-Yaacov, Yonina C Eldar
Aug 30, 2008·PloS One·Nora Khaldi, Kenneth H Wolfe
Sep 13, 2008·PLoS Pathogens·Andrew McDonaghElaine Bignell
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Stéphane Le CromAntoine Margeot

❮ Previous
Next ❯

Citations

Aug 7, 2013·Annual Review of Phytopathology·Prasun K MukherjeeCharles M Kenerley
Dec 22, 2011·BMC Genomics·Mikko ArvasMarkku Saloheimo
Jan 4, 2012·Biotechnology for Biofuels·André SchusterMonika Schmoll
Jul 20, 2014·Briefings in Functional Genomics·María Victoria Aguilar-PontesMiaomiao Zhou
Oct 25, 2014·Biotechnology for Biofuels·Thiago M Mello-de-SousaAstrid R Mach-Aigner
Dec 3, 2014·Enzyme and Microbial Technology·Yuhong HuangLene Lange
Aug 14, 2012·Fungal Genetics and Biology : FG & B·Christian SeibelMonika Schmoll
Jun 25, 2013·Fungal Genetics and Biology : FG & B·Michel FlipphiLevente Karaffa
Jan 30, 2018·Frontiers in Microbiology·Eva StapplerMonika Schmoll
May 10, 2020·Genetics and Molecular Biology·Leonardo Martins-SantanaRafael Silva-Rocha
Aug 6, 2018·Applied Microbiology and Biotechnology·Scott E Baker
Jun 4, 2018·Applied Microbiology and Biotechnology·Janina KlugeUlrich Kück
Jun 16, 2017·Biotechnology for Biofuels·Etienne JourdierFrédérique Bidard

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE19606

Methods Mentioned

BETA
irradiating
chip
PCR
GTPase
Illumina sequencing
protein assay

Software Mentioned

Limma
snapCGH
DNAcopy
R
Bioconductor
Ringo
RocheNimbleGen
Primer3

Related Concepts

Related Feeds

Cardiac Glycosides

Cardiac glycosides are a diverse family of naturally derived compounds that bind to and inhibit na+/k+-atpase. Discover the latest research on cardiac glycosides heres.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.