Jul 8, 2016

Arrays of microscopic organic LEDs for high-resolution optogenetics

Science Advances
Anja SteudeMalte C Gather

Abstract

Optogenetics is a paradigm-changing new method to study and manipulate the behavior of cells with light. Following major advances of the used genetic constructs over the last decade, the light sources required for optogenetic control are now receiving increased attention. We report a novel optogenetic illumination platform based on high-density arrays of microscopic organic light-emitting diodes (OLEDs). Because of the small dimensions of each array element (6 × 9 μm(2)) and the use of ultrathin device encapsulation, these arrays enable illumination of cells with unprecedented spatiotemporal resolution. We show that adherent eukaryotic cells readily proliferate on these arrays, and we demonstrate specific light-induced control of the ionic current across the membrane of individual live cells expressing different optogenetic constructs. Our work paves the way for the use of OLEDs for cell-specific optogenetic control in cultured neuronal networks and for acute brain slices, or as implants in vivo.

Mentioned in this Paper

Led-CC-I peptide
In Vivo
Membrane
Anatomic Structures
Neurons
Brain
Implants
Ion Channel
Neuronal
Optogenetics

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