PMID: 7371065May 1, 1980Paper

Arrested growth of Chinese hamster ovary (CHO) cell colonies on agar

Cell and Tissue Kinetics
B StorrieD A Glaser

Abstract

Chinese hamster ovary (CHO) cells plated on agar form two classes of colonies; those which increase continuously in diameter and those which become arrested in outward growth. All colonies continue to increase in mass and thickness as demonstrated by computer-assisted analysis of time sequence photographs of several thousand colonies and by examination of histological sections. Colonies which shift from a monolayer to a mounded morphology at fairly large colony diameter (greater than or approximately 1 mm) continue to increase in diameter. Colonies in which mounding occurs at smaller colony diameter (less than or approximately 1 mm) cease to increase in diameter but continue to increase in thickness, as demonstrated by histological examination and by computer-assisted analysis. Rapid cell division occurs at the edge of all colony classes, as shown by the distribution of mitotic figures. In arrested colonies these dividing cells must move toward the colony core to compensate for dying cells. Necrotic cells are found as a discrete zone at the air-colony interface in all cases for CHO cell colonies growing on agar. As such, necrosis is probably due to limitations in nutrient diffusion upward from the agar rather than oxygen diffus...Continue Reading

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·B D AidellsD A Glaser
Dec 31, 1975·Biochimica Et Biophysica Acta·J Folkman, H P Greenspan
Jul 15, 1977·International Journal of Cancer. Journal International Du Cancer·J Carlsson
Sep 1, 1976·Cell and Tissue Kinetics·R E Durand
Mar 31, 1972·Science·N M Braslow, R L Bowman
Jan 1, 1973·Methods in Cell Biology·L H Thompson, R M Baker
Oct 1, 1973·The Journal of Experimental Medicine·J Folkman, M Hochberg

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Citations

Aug 1, 1980·Computers and Biomedical Research, an International Journal·M W KonradD A Glaser
Jun 1, 1982·British Journal of Addiction·F B Glaser, A C Ogborne
Mar 1, 1982·British Journal of Addiction·H A SkinnerH M Annis
Dec 1, 1981·British Journal of Addiction·A K Cartwright

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