Arrestin with a single amino acid substitution quenches light-activated rhodopsin in a phosphorylation-independent fashion

Biochemistry
M P Gray-KellerV V Gurevich

Abstract

Arrestins are members of a superfamily of regulatory proteins that participate in the termination of G protein-mediated signal transduction. In the phototransduction cascade of vertebrate rods, which serves as a prototypical G protein-mediated signaling pathway, the binding of visual arrestin is stimulated by phosphorylation of the C-terminus of photoactivated rhodopsin (Rh*). Arrestin is very selective toward light-activated phosphorhodopsin (P-Rh*). Previously we reported that a single amino acid substitution in arrestin, Arg175Gln, results in a dramatic increase in arrestin binding to Rh* [Gurevich, V. V., & Benovic, J. L. (1995) J. Biol. Chem. 270, 6010-6016]. Here we demonstrate that a similar mutant, arrestin(R175E), binds to light-activated rhodopsin independent of phosphorylation. Arrestin(R175E) binds with high affinity not only to P-Rh* and Rh* but also to light-activated truncated rhodopsin in which the C-terminus phosphorylation sites have been proteolytically removed. In an in vitro assay that monitored rhodopsin-dependent activation of cGMP phosphodiesterase (PDE), wild type arrestin quenched PDE response only when ATP was present to support rhodopsin phosphorylation. In contrast, as little as 30 nM arrestin(R175E...Continue Reading

Citations

Apr 24, 2010·Expert Reviews in Molecular Medicine·Vsevolod V Gurevich, Eugenia V Gurevich
Feb 4, 2011·Biochemistry·Miyeon KimVsevolod V Gurevich
Aug 3, 2002·European Journal of Biochemistry·Brian H ShiltonPaul A Hargrave
Jan 26, 2012·The Journal of Biological Chemistry·Luis E GimenezVsevolod V Gurevich
Jul 13, 2012·The Journal of Biological Chemistry·Luis E GimenezVsevolod V Gurevich
Dec 13, 2005·The Journal of Biological Chemistry·Susan M Hanson, Vsevolod V Gurevich
Sep 13, 2007·The Journal of Biological Chemistry·Sergey A VishnivetskiyVsevolod V Gurevich
Mar 15, 2012·American Journal of Physiology. Endocrinology and Metabolism·Hesham A Tawfeek, Abdul B Abou-Samra
Mar 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Susan M HansonVsevolod V Gurevich
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·S MukherjeeM Hunzicker-Dunn
Mar 12, 2013·The Journal of Biological Chemistry·Sergey A VishnivetskiyVsevolod V Gurevich
Apr 4, 2000·FEBS Letters·T MaedaA Murakami
Oct 12, 2010·Critical Reviews in Biochemistry and Molecular Biology·Laura M Bohn, Cullen L Schmid
Jun 6, 2012·Cellular Signalling·Vsevolod V Gurevich, Eugenia V Gurevich
Feb 8, 2006·Pharmacology & Therapeutics·Vsevolod V Gurevich, Eugenia V Gurevich
Jan 13, 2015·British Journal of Pharmacology·E V Gurevich, V V Gurevich
Sep 10, 2013·Cellular Signalling·Xiufeng SongVsevolod V Gurevich
Aug 10, 2011·Progress in Retinal and Eye Research·Vsevolod V GurevichEugenia V Gurevich
Aug 1, 2015·Genes & Diseases·Henriette Stoy, Vsevolod V Gurevich
Dec 3, 2011·Protein Expression and Purification·Li HuangKevin D Ridge
Jul 17, 1998·Vision Research·J B HurleyG A Niemi
Mar 8, 2005·Biophysical Journal·Candice S KlugJames S Hyde
Dec 30, 2014·Biochemical and Biophysical Research Communications·Youngjoo YunKa Young Chung
Oct 30, 2015·Scientific Reports·Joachim GranzinRenu Batra-Safferling
May 26, 2017·The Journal of Biological Chemistry·Sergey A VishnivetskiyVsevolod V Gurevich
Sep 7, 2002·The Journal of Biological Chemistry·Sergey A VishnivetskiyVsevolod V Gurevich
Apr 17, 1999·The Journal of Biological Chemistry·S A VishnivetskiyV V Gurevich
Jan 26, 2002·The Journal of Biological Chemistry·Astra DinculescuW Clay Smith
Sep 2, 2000·The Journal of Biological Chemistry·A PulvermüllerK P Hofmann
Jan 10, 2002·The Journal of Biological Chemistry·Jeremy CelverVsevolod V Gurevich
Jan 15, 2003·The Journal of Biological Chemistry·Ling PanVsevolod V Gurevich
Oct 12, 2000·The Journal of Biological Chemistry·S A VishnivetskiyV V Gurevich
Jul 6, 2018·Frontiers in Molecular Neuroscience·Srimal SamaranayakeVsevolod V Gurevich
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·A E Otto-BrucK Palczewski
Jul 1, 2020·Journal of Neurochemistry·Sergey A VishnivetskiyVsevolod V Gurevich
Nov 20, 1997·The Journal of Biological Chemistry·V V GurevichJ J Onorato
Aug 18, 2020·Archives of Pharmacal Research·Kiae Kim, Ka Young Chung
Jul 20, 1999·The Journal of Biological Chemistry·C SchubertK G Fleming
Aug 6, 2020·The Journal of Biological Chemistry·Ya ZhuoAdriano Marchese
Nov 8, 2020·Journal of Neurochemistry·Sergey A VishnivetskiyVsevolod V Gurevich

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.