Arsenic Induces Thioredoxin 1 and Apoptosis in Human Liver HHL-5 Cells

Biological Trace Element Research
Yuanyuan LiYudan Wei

Abstract

To further characterize the mechanisms underlying liver toxicity induced by arsenic, we examined in this study the effect of arsenic on thioredoxin (Trx) and the apoptotic signaling pathways in human liver HHL-5 cells. The cells were treated with 0, 2, 5, and 10 μM of sodium arsenite for 24 h, and the changes of Trx1 and thioredoxin reductase (TrxR1) as well as intracellular ROS and apoptosis were examined. A concentration-dependent increase in mRNA and protein levels of Trx1 and TrxR1 was observed in arsenic-treated cells. Intracellular ROS levels and apoptosis were also significantly increased in a concentration-dependent manner. In line with this, protein levels of Bax and cytochrome C were increased and Bcl-2 was decreased by arsenic treatments. Increases in caspase 3 activity were observed. These results indicate that Trx is involved in arsenic-induced liver cell injury, probably through the apoptotic signaling pathway. However, further studies are needed to elucidate on these findings.

References

Apr 4, 2000·Physiological Reviews·S Y Hsu, A J Hsueh
Jun 22, 2005·Toxicology and Applied Pharmacology·D N Guha Mazumder
Jun 22, 2005·Toxicology and Applied Pharmacology·Chien-Jen ChenMeei-Maan Wu
Jun 28, 2005·Toxicology and Applied Pharmacology·Juanita BustamanteVladimir Gogvadze
Jan 22, 2008·Biochimica Et Biophysica Acta·Nilanjana Maulik, Dipak K Das
Nov 4, 2008·Molecular Nutrition & Food Research·Kathryn F Tonissen, Giovanna Di Trapani
Jul 19, 2011·Journal of Molecular Graphics & Modelling·Fangfang WangYonghua Wang
Oct 11, 2011·Toxicology and Applied Pharmacology·Min TanGavin E Arteel
Dec 29, 2011·Toxicology and Applied Pharmacology·Yuanyuan LiDianjun Sun
Mar 6, 2013·Environmental Health Perspectives·Marisa F NaujokasWilliam A Suk
Sep 24, 2013·Biological Trace Element Research·Xiaona LiuJing Wang
Jun 25, 2014·Toxicology Letters·Cheng WangDian-Jun Sun
Aug 1, 2014·Environmental Toxicology·Xiao PanXiance Sun
Oct 7, 2014·Journal of Preventive Medicine and Public Health = Yebang Ŭihakhoe Chi·Young-Seoub HongJin-Yong Chung
Aug 21, 2015·Environmental Toxicology and Pharmacology·Xiao-Feng YaoXian-Ce Sun
Feb 11, 2016·International Journal of Environmental Research and Public Health·Hui ShenMingxia Jing
Oct 11, 2016·The Journal of Nutritional Biochemistry·Sreetama ChoudhurySreya Chattopadhyay
Mar 7, 2017·Biological Trace Element Research·Hongyu ChenZunzhen Zhang

❮ Previous
Next ❯

Citations

Oct 12, 2018·Environmental Science and Pollution Research International·Marzieh Zeinvand-LorestaniLayasadat Khorsandi
Apr 12, 2020·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Tianxiao DuanPeng Luo
Dec 23, 2020·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Mengke YuanJundong Wang

❮ Previous
Next ❯

Methods Mentioned

BETA
protein assay
PCR
Infrared Imaging
flow cytometry
transmission electron microscopy

Software Mentioned

SPSS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.