PMID: 39620Sep 28, 1979

Arsenite inhibits beta-oxidation in isolated rat liver mitochondria

Biochimica Et Biophysica Acta
K A ReinJ Bremer

Abstract

A partial inhibition of acylcarnitine oxidation by arsenite in rat liver mitochondria has been studied. This inhibition is confined to the thiolase(s). The inhibition was observed also in the presence of malate, indicating no selective effect on ketogenesis. Ketogenesis from acetyl-CoA was inhibited by arsenite. Mitochondrial CoA was acylated by acylcarnitine nearly as rapidly in the presence of arsenite as in its absence. Thus, arsenite did not interfere with the availibility of CoA in the mitochondria. No effect of arsenite on enzymes of beta-oxidation other than the thiolase(s) was observed. When arsenite and acylcarnitine were added simultaneously to mitochondria, there was a delay before maximal inhibition of oxygen uptake occurred. When the mitochondria were preincubated with arsenite before addition of acylcarnitine, the inhibitory effect on oxygen utilization was initially large, but then partially repealed. Similar time delays were observed in the activity of acetoacetyl-CoA thiolase of disrupted mitochondria depending on the sequence of arsenite and acetoacetyl-CoA addition. It is suggested that substrate and arsenite complete for the reactive sulfhydryl group at the active site of the thiolase(s).

References

Sep 1, 1953·Biochimica Et Biophysica Acta·F LYNEN, S OCHOA

Citations

Jan 1, 1988·Archives of Toxicology·L Szinicz, W Forth
Aug 1, 1984·The Science of the Total Environment·J Z Byczkowski, J R Sorenson
Jan 1, 1981·Comparative Biochemistry and Physiology. C: Comparative Pharmacology·N FuentesM Rosenmann
Apr 24, 2008·Metal-based Drugs·Stephen John Ralph
Mar 16, 1982·Biochimica Et Biophysica Acta·M MarkefskiV P Zinchenko
Jun 28, 1996·Journal of Toxicology and Environmental Health·S L ShalatR H Finnell
Jun 1, 1981·Bulletin of Environmental Contamination and Toxicology·M N BaxleyG M Szczech
Dec 1, 1995·Scandinavian Journal of Gastroenterology·J AasethE Schrumpf

Related Concepts

Acetyl-CoA C-Acetyltransferase
Arsenic
Carnitine Palmitoyltransferase II
CoASH
Mitochondria, Liver

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