Arsenite response in Coccomyxa sp. Carn explored by transcriptomic and non-targeted metabolomic approaches

Environmental Microbiology
Sandrine KoechlerDavid Halter

Abstract

Arsenic is a toxic metalloid known to generate an important oxidative stress in cells. In the present study, we focused our attention on an alga related to the genus Coccomyxa, exhibiting an extraordinary capacity to resist high concentrations of arsenite and arsenate. The integrated analysis of high-throughput transcriptomic data and non-targeted metabolomic approaches highlighted multiple levels of protection against arsenite. Indeed, Coccomyxa sp. Carn induced a set of transporters potentially preventing the accumulation of this metalloid in the cells and presented a distinct arsenic metabolism in comparison to another species more sensitive to that compound, i.e. Euglena gracilis, especially in regard to arsenic methylation. Interestingly, Coccomyxa sp. Carn was characterized by a remarkable accumulation of the strong antioxidant glutathione (GSH). Such observation could explain the apparent low oxidative stress in the intracellular compartment, as suggested by the transcriptomic analysis. In particular, the high amount of GSH in the cell could play an important role for the tolerance to arsenate, as suggested by its partial oxidation into oxidized glutathione in presence of this metalloid. Our results therefore reveal that...Continue Reading

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Citations

Jul 3, 2018·Chemical & Pharmaceutical Bulletin·Shizuyo HoriyamaMasaru Kunitomo
Dec 22, 2019·Metabolomics : Official Journal of the Metabolomic Society·Léa GauthierNicolas Mazzella
Nov 2, 2017·Environmental Science and Pollution Research International·Florence Arsène-PloetzeValérie Barbe
Sep 10, 2020·Microorganisms·Diana Ayala-MuñozJennifer L Macalady
Sep 13, 2017·Proceedings of the National Academy of Sciences of the United States of America·Shunsuke HirookaShin-Ya Miyagishima
Oct 6, 2020·Frontiers in Bioengineering and Biotechnology·Gulshan KumarTilak Raj Sharma
May 12, 2021·Environmental Microbiology Reports·Frédéric PlewniakPhilippe Bertin
Jun 1, 2021·Extremophiles : Life Under Extreme Conditions·Giacomo FaisGiacomo Cao
Jun 6, 2021·Environmental Pollution·Shweta Tripathi, Krishna Mohan Poluri

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