ArsH is an organoarsenical oxidase that confers resistance to trivalent forms of the herbicide monosodium methylarsenate and the poultry growth promoter roxarsone

Molecular Microbiology
Jian ChenBarry P Rosen

Abstract

Environmental organoarsenicals are produced by microorganisms and are introduced anthropogenically as herbicides and antimicrobial growth promoters for poultry and swine. Nearly every prokaryote has an ars (arsenic resistance) operon, and some have an arsH gene encoding an atypical flavodoxin. The role of ArsH in arsenic resistance has been unclear. Here we demonstrate that ArsH is an organoarsenical oxidase that detoxifies trivalent methylated and aromatic arsenicals by oxidation to pentavalent species. Escherichia coli, which does not have an arsH gene, is very sensitive to the trivalent forms of the herbicide monosodium methylarsenate [MSMA or MAs(V)] and antimicrobial growth promoter roxarsone [Rox(V)], as well as to phenylarsenite [PhAs(III), also called phenylarsine oxide or PAO]. Pseudomonas putida has two chromosomally encoded arsH genes and is highly resistant to the trivalent forms of these organoarsenicals. A derivative of P. putida with both arsH genes deleted is sensitive to MAs(III), PhAs(III) or Rox(III). P. putida arsH expressed in E. coli conferred resistance to each trivalent organoarsenical. Cells expressing PpArsH oxidized the trivalent organoarsenicals. PpArsH was purified, and the enzyme in vitro similarly...Continue Reading

References

Feb 1, 1995·Journal of Bacteriology·A CarlinB P Rosen
Feb 1, 1997·Journal of Bacteriology·C NeytG R Cornelis
Aug 2, 2002·Plasmid·David Ryan, Emer Colleran
Sep 2, 2003·Journal of Bacteriology·Luis López-MauryJosé C Reyes
Dec 4, 2003·Environmental Microbiology·David CánovasVíctor de Lorenzo
Oct 4, 2005·Journal of Bacteriology·Hung-Chi YangHiranmoy Bhattacharjee
Feb 3, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jie QinChristopher Rensing
May 18, 2006·Annual Review of Microbiology·John F StolzRonald S Oremland
Aug 4, 2007·FEBS Letters·Jun YeHiranmoy Bhattacharjee
Mar 6, 2009·Organic & Biomolecular Chemistry·Nicole Jasmin MuellerKurt Faber
Mar 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jie QinBarry P Rosen
Jan 29, 2011·Environmental Microbiology·Masafumi YoshinagaBarry P Rosen
Feb 16, 2011·Journal of Applied Toxicology : JAT·K JomovaM Valko
Jan 20, 2012·Trends in Plant Science·Jun YeYong-Guan Zhu
Oct 11, 2012·Current Topics in Membranes·Hung-Chi YangBarry P Rosen
Dec 24, 2013·Environmental Science & Technology·Jian ChenBarry P Rosen
Dec 24, 2013·Archives of Biochemistry and Biophysics·Mieke M E HuijbersWillem J H van Berkel
Mar 29, 2014·Environmental Microbiology·A David Páez-EspinoVíctor de Lorenzo
May 14, 2014·Proceedings of the National Academy of Sciences of the United States of America·Masafumi Yoshinaga, Barry P Rosen
May 28, 2014·FEMS Microbiology Letters·Xi-Mei XueJun Ye
May 1, 2014·Annual Review of Earth and Planetary Sciences·Yong-Guan ZhuBarry P Rosen

❮ Previous
Next ❯

Citations

Feb 15, 2016·Current Opinion in Biotechnology·David C Gillan
Jan 23, 2016·FEMS Microbiology Reviews·Jérémy Andres, Philippe N Bertin
Nov 7, 2015·Environmental Science & Technology·Yu YanYong-Guan Zhu
Aug 4, 2015·Molecular Microbiology·Jian ChenBarry P Rosen
Apr 24, 2016·Biomedical Journal·Hung-Chi Yang, Barry P Rosen
Jul 2, 2016·Environmental Science & Technology·Jian Chen, Barry P Rosen
Apr 3, 2016·Applied and Environmental Microbiology·Yoon-Suk KangTimothy R McDermott
May 22, 2016·Applied and Environmental Microbiology·Matilde FernándezTino Krell
Nov 28, 2019·Environmental Microbiology Reports·Timothy R McDermottRonald S Oremland
Mar 24, 2020·Environmental Microbiology·A David Páez-EspinoVíctor de Lorenzo
Sep 12, 2018·World Journal of Microbiology & Biotechnology·Nallely Serrato-GamiñoCarlos Cervantes
Sep 2, 2017·Molecular Microbiology·Jian ChenBarry P Rosen
May 28, 2019·Frontiers in Microbiology·Andrea FirrincieliMartina Cappelletti
May 1, 2020·Proceedings of the National Academy of Sciences of the United States of America·Song-Can ChenYong-Guan Zhu
Nov 9, 2018·Frontiers in Microbiology·Ibtissem Ben FekihCarlos Cervantes
Jan 14, 2020·Current Pharmacology Reports·Michael CoryellSeth T Walk
Nov 30, 2019·Environmental Science and Pollution Research International·Mariana Elisa VezzaMelina Andrea Talano
Dec 7, 2018·Molecular Microbiology·Jian ChenBarry P Rosen
Oct 13, 2016·Metallomics : Integrated Biometal Science·Jiaojiao LiBarry P Rosen
Sep 5, 2020·Molecular Biology and Evolution·Fatima Foflonker, Crysten E Blaby-Haas
Jan 5, 2021·Heliyon·Marcos PileggiMichael J Sadowsky
Jan 12, 2021·Computational and Structural Biotechnology Journal·Xiuli HaoYu-Rong Liu
Feb 23, 2021·Extremophiles : Life Under Extreme Conditions·Carlos Eduardo BarragánDolly Montoya
Jan 30, 2021·FEMS Microbiology Ecology·Almudena Escobar-NiñoDavid Cánovas
Aug 19, 2020·The Science of the Total Environment·Jian Chen, Barry P Rosen
Apr 15, 2021·Current Environmental Health Reports·Yifei YangKun Lu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Related Papers

Environmental Science & Technology
Jian ChenBarry P Rosen
Proceedings of the National Academy of Sciences of the United States of America
Masafumi Yoshinaga, Barry P Rosen
Applied and Environmental Microbiology
Jian ChenBarry P Rosen
Acta Crystallographica. Section D, Biological Crystallography
Kavitha MarapakalaBarry P Rosen
© 2022 Meta ULC. All rights reserved