Artefacts at the liquid interface and their impact in miniaturized biochemical assay

Biomicrofluidics
Brett LittenPeter Fielden

Abstract

Droplet microfluidic technology has the potential to significantly reduce reagent use, and therefore, lower costs of assays employed in drug discovery campaigns. In addition to the reduction in costs, this technology can also reduce evaporation and contamination which are often problems seen in miniaturized microtitre plate formats. Despite these advantages, we currently advise caution in the use of these microfluidic approaches as there remains a lack of understanding of the artefacts of the systems such as reagent partitioning from droplet to carrier oil and interaction of the biological reagents with the water-oil interface. Both types of artefact can lead to inaccurate and misleading data. In this paper, we present a study of the partitioning of a number of drug-like molecules in a range of oils and evidence of protein binding at the water-oil interface which results in reduced activity of a cytochrome P450 enzyme. Data presented show that the drug-like molecules partitioned the least into fluorocarbon oils and the interaction of the 1A2 cytochrome at the water-oil interface resulted in a lower or complete absence of enzyme activity. This loss of activity of cytochrome 1A2 could be restored by the use of secondary blocking ...Continue Reading

References

Nov 12, 2002·Methods in Enzymology·Charles L CrespiDavid M Stresser
Sep 21, 2004·Biomedical Microdevices·Guillaume Tresset, Shoji Takeuchi
Mar 31, 2005·Annual Review of Physical Chemistry·Matthew A Holden, Paul S Cremer
Jun 2, 2006·Lab on a Chip·Wei-Heong Tan, Shoji Takeuchi
Oct 21, 2011·Lab on a Chip·Jean-Christophe Baret
Oct 24, 2012·Lab on a Chip·Yunhan ChenSindy K Y Tang

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Citations

Feb 13, 2016·Biomicrofluidics·P EribolK O Ulgen
Oct 1, 2015·Biomicrofluidics·Brigitte Stadler
Feb 18, 2017·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Nauman KhalidMitsutoshi Nakajima

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