Artemisia capillaris inhibited enterovirus 71-induced cell injury by preventing viral internalization

The Kaohsiung Journal of Medical Sciences
Ming-Hong YenJung San Chang

Abstract

Artemisia capillaris (A. capillaris) is a common herbal drug used for thousands years in ancient China. A. capillaris has been empirically used to manage hand-foot-mouth disease (HFMD), which is commonly caused by enterovirus 71 (EV71). EV71 can cause meningoencephalitis with mortality and neurologic sequelae without effective management. It is presently unknown whether A. capillaris is effective against EV71 infection. To test the hypothesis that it could protect cells from EV71-induced injury, a hot water extract of A. capillaris was tested in human foreskin fibroblast cells (CCFS-1/KMC) and human rhabdomyosarcoma cells (RD cells) by plaque reduction assay and flow cytometry. Inhibition of viral replication was examined by reverse quantitative RT-PCR (qRT-PCR). Its effect on translations of viral proteins (VP0, VP1, VP2, protease 2B and 3AB), and apoptotic proteins were examined by western blot. A. capillaris was dose-dependently effective against EV71 infection in both CCFS-1/KMC cells and RD cells by inhibiting viral internalization. However, A. capillaris was minimally effective on viral attachment, VP2 translation, and inhibition of virus-induced apoptosis. Further isolation of effective molecules is needed. In conclusion...Continue Reading

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Citations

Aug 20, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Jiayu ZhongHong Cao
Apr 16, 2020·BMC Complementary Medicine and Therapies·Salima S LalaniChit Laa Poh
Aug 7, 2021·Journal of the Science of Food and Agriculture·Su Jung HwangHyo-Jong Lee

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