Artemisinin Represses Telomerase Subunits and Induces Apoptosis in HPV-39 Infected Human Cervical Cancer Cells

Journal of Cellular Biochemistry
Anushree Mondal, Urmi Chatterji

Abstract

Artemisinin, a plant-derived antimalarial drug with relatively low toxicity on normal cells in humans, has selective anticancer activities in various types of cancers, both in vitro and in vivo. In the present study, we have investigated the anticancer effects of artemisinin in human cervical cancer cells, with special emphasis on its role in inducing apoptosis and repressing cell proliferation by inhibiting the telomerase subunits, ERα which is essential for maintenance of the cervix, and downstream components like VEGF, which is known to activate angiogenesis. Effects of artemisinin on apoptosis of ME-180 cells were measured by flow cytometry, DAPI, and annexin V staining. Expression of genes and proteins related to cell proliferation and apoptosis was quantified both at the transcriptional and translational levels by semi-quantitative RT-PCR and western blot analysis, respectively. Our findings demonstrated that artemisinin significantly downregulated the expression of ERα and its downstream component, VEGF. Antiproliferative activity was also supported by decreased telomerase activity and reduced expression of hTR and hTERT subunits. Additionally, artemisinin reduced the expression of the HPV-39 viral E6 and E7 components. ...Continue Reading

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Citations

Oct 13, 2015·Expert Opinion on Investigational Drugs·Lynne HampsonIan N Hampson
Oct 27, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Danfeng WangYuxin Li
Sep 3, 2019·Current Pharmaceutical Design·Maushmi S KumarMayur C Yergeri
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Apr 11, 2018·Natural Products and Bioprospecting·Yunqin ZhangZhili Zuo
Jul 19, 2020·International Journal of Molecular Sciences·Axelle Septembre-MalaterrePhilippe Gasque
Mar 7, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cristina Adriana DeheleanIulia Pinzaru
Mar 2, 2021·Chinese Journal of Natural Medicines·Se-Eun LeeSuin Cho
Jun 21, 2021·Medicinal Research Reviews·Yuqing MengJigang Wang

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