Arterial Calcification Is Regulated Via an miR-204/DNMT3a Regulatory Circuit Both In Vitro and in Female Mice

Endocrinology
Xiao LinLing-Qing Yuan

Abstract

Arterial calcification is a common cardiovascular disease that initiates from a process of osteoblastic differentiation of vascular smooth muscle cells (VSMCs). Accumulating evidence has demonstrated that microRNAs play an important role in regulating arterial calcification. miR-204 was significantly downregulated in calcified human renal arteries from patients with uremia; calcified arteries of mice, due to 5/6 nephrectomy with a high-phosphate diet (5/6 NTP); and in VSMCs induced by high phosphate concentration. The overexpression of miR-204 alleviated the osteoblastic differentiation of VSMCs. Bisulphite sequencing PCR revealed that CpG sites upstream of miR-204 DNA were hypermethylated in calcified VSMCs; in calcified arteries of mice, due to 5/6 NTP; and in calcified renal artery tissues from patients with uremia. Moreover, increased DNMT3a resulted in the hypermethylation of miR-204 in high phosphate concentration-induced VSMCs, whereas 5-aza-2'-deoxycytidine could restore the expression of miR-204 in high phosphate concentration-induced VSMCs. Moreover, we found that DNMT3a was the target of miR-204, and the methylation ratio of miR-204 was decreased significantly, meaning that the expression of miR-204 was restored when...Continue Reading

References

May 18, 2000·The New England Journal of Medicine·W G GoodmanI B Salusky
Sep 27, 2000·Human Molecular Genetics·T H Bestor
Apr 15, 2003·Toxicology and Applied Pharmacology·Tsung-Ming ShihJohn H McDonough
Jun 3, 2005·Current Opinion in Nephrology and Hypertension·Dominique PriéGérard Friedlander
Sep 2, 2005·Nature Reviews. Genetics·Keith D Robertson
Feb 24, 2006·Nature Reviews. Cancer·Stephen B Baylin, Joyce E Ohm
Jan 17, 2008·Nature Reviews. Genetics·Witold FilipowiczNahum Sonenberg
May 26, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Addy Montes de OcaEscolastico Aguilera-Tejero
Mar 20, 2012·Cell·Joshua T Mendell, Eric N Olson
Aug 9, 2012·Cardiovascular Research·Rong-Rong CuiEr-Yuan Liao
Jun 27, 2013·Endocrinology·Xiao-Bo LiaoLing-Qing Yuan
Jul 26, 2013·Kidney International·Yifu FangKeith A Hruska
Sep 7, 2013·Endocrinology·Denuja Karunakaran, Katey J Rayner
Apr 24, 2014·Handbook of Experimental Pharmacology·Johannes Oberwinkler, Stephan E Philipp
Jun 25, 2014·The Journal of Clinical Investigation·Thomas BerteroStephen Y Chan
Mar 7, 2015·The Journal of Toxicological Sciences·Seong-Ho HongMyung-Haing Cho
Jul 19, 2015·Journal of the American Society of Nephrology : JASN·Sara PanizoIsabel Rodríguez
May 6, 2016·American Journal of Respiratory and Critical Care Medicine·Grégoire RuffenachSébastien Bonnet
Jun 16, 2017·Scientific Reports·Yi-Qun PengLing-Qing Yuan

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Citations

Mar 15, 2019·Endocrine-related Cancer·Feng WuZhaohui Mo
Nov 15, 2019·Current Pharmaceutical Design·Chao TuZhihong Li
May 8, 2020·International Journal of Molecular Sciences·Duk-Hwa KwonHyun Kook
Nov 30, 2019·Journal of Translational Medicine·Alejandra Tapia-CastilloCristian A Carvajal
Feb 7, 2020·International Journal of Molecular Sciences·Yi-Chou HouKuo-Cheng Lu
Oct 23, 2020·Journal of Cellular and Molecular Medicine·Shan-Shan WangHan Chen
Sep 11, 2020·Pharmacology & Therapeutics·Juhee RyuYoung-Kook Kim
Oct 24, 2020·Clinica Chimica Acta; International Journal of Clinical Chemistry·Meng DuanKai Yin
Oct 24, 2020·Clinica Chimica Acta; International Journal of Clinical Chemistry·Meng DuanKai Yin
Dec 1, 2021·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Yaling BaiJinsheng Xu

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