Artery targeted photothrombosis widens the vascular penumbra, instigates peri-infarct neovascularization and models forelimb impairments

Scientific Reports
Taylor A ClarkTheresa A Jones

Abstract

The photothrombotic stroke model generates localized and reproducible ischemic infarcts that are useful for studying recovery mechanisms, but its failure to produce a substantial ischemic penumbra weakens its resemblance to human stroke. We examined whether a modification of this approach, confining photodamage to arteries on the cortical surface (artery-targeted photothrombosis), could better reproduce aspects of the penumbra. Following artery-targeted or traditional photothrombosis to the motor cortex of mice, post-ischemic cerebral blood flow was measured using multi-exposure speckle imaging at 6, 48, and 120 h post-occlusion. Artery-targeted photothrombosis produced a more graded penumbra at 48 and 120 h. The density of isolectin B4+ vessels in peri-infarct cortex was similarly increased after both types of infarcts compared to sham at 2 weeks. These results indicate that both models instigated post-ischemic vascular structural changes. Finally, we determined whether the strength of the traditional photothrombotic approach for modeling upper-extremity motor impairments extends to the artery-targeted approach. In adult mice that were proficient in a skilled reaching task, small motor-cortical infarcts impaired skilled-reachi...Continue Reading

References

Jan 1, 1977·Stroke; a Journal of Cerebral Circulation·J AstrupN A Lassen
Apr 1, 1986·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·W D DietrichB D Watson
May 1, 1985·Annals of Neurology·B D WatsonM D Ginsberg
Nov 1, 1981·Stroke; a Journal of Cerebral Circulation·J AstrupL Symon
Sep 1, 1994·Stroke; a Journal of Cerebral Circulation·J KrupinskiJ M Wang
Apr 1, 1997·Stroke; a Journal of Cerebral Circulation·M Fisher
Oct 6, 2000·The Journal of Clinical Investigation·Z G ZhangM Chopp
Sep 8, 2001·Stroke; a Journal of Cerebral Circulation·L WeiT A Woolsey
Oct 13, 2001·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·P S ManoonkitiwongsaP D Lyden
Feb 7, 2003·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Takeshi HayashiPak H Chan
Dec 25, 2003·Cerebrovascular Diseases·Marc Fisher
Aug 7, 2004·Brain Research. Brain Research Protocols·Hiroshi SugimoriMitsuo Iida
Jan 4, 2006·NeuroRx : the Journal of the American Society for Experimental NeuroTherapeutics·S Thomas Carmichael
Dec 15, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·John J OhabS Thomas Carmichael
Dec 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Nozomi NishimuraDavid Kleinfeld
Apr 13, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Craig E BrownTimothy H Murphy
Jul 3, 2007·Current Treatment Options in Cardiovascular Medicine·Juan F ArenillasAntoni Dávalos
Aug 23, 2007·ILAR Journal·Jeffrey A KleimDeAnna L Adkins
Sep 1, 2007·Stroke; a Journal of Cerebral Circulation·Tomás SobrinoJosé Castillo
Jan 23, 2008·Expert Opinion on Therapeutic Targets·Iordanis Gravanis, Stella E Tsirka
Feb 22, 2008·Journal of Neuroscience Methods·Albrecht SiglerTimothy H Murphy
May 15, 2008·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Chien-Yuan LinTeng-Nan Lin
Aug 14, 2008·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Akihiko TaguchiHiroaki Naritomi
Nov 6, 2009·Nature Reviews. Neuroscience·Timothy H Murphy, Dale Corbett
Nov 18, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Nick M E A HaywardJukka Jolkkonen
Jan 25, 2011·Biomedical Optics Express·Ashwin B ParthasarathyAndrew K Dunn
Apr 13, 2011·Brain : a Journal of Neurology·Steven C CramerSophia Vinogradov
Jun 17, 2011·Developmental Psychobiology·Theresa A Jones, Stephanie C Jefferson
Dec 28, 2011·Drug Delivery and Translational Research·Hayder JafferVinod Labhasetwar
Apr 3, 2012·Neurorehabilitation and Neural Repair·John W KrakauerGeorge F Wittenberg
Apr 12, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Abraham MartínBertrand Tavitian
Nov 16, 2012·Journal of Neuroscience Research·Y AvrahamR R Leker
Feb 7, 2013·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Andrew N ClarksonS Thomas Carmichael
Apr 11, 2013·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Syed Mohammad Shams KazmiAndrew K Dunn
Aug 8, 2013·Stroke; a Journal of Cerebral Circulation·Roshini PrakashAdviye Ergul

❮ Previous
Next ❯

Citations

Feb 12, 2020·Neurophotonics·Smrithi SunilKıvılcım Kılıç
Mar 13, 2020·Nature Methods·Mihail Ivilinov TodorovAli Ertürk
Apr 29, 2021·Cell Reports·Michael R WilliamsonTheresa A Jones
Aug 10, 2021·Current Protocols·Jacqueline M GarrickJudit Marsillach
Oct 9, 2021·PLoS Computational Biology·Samuel A MihelicAndrew K Dunn

❮ Previous
Next ❯

Software Mentioned

Neurolucida TM
SPSS
MATLAB
ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Neuroscience Methods
Ursula I TuorMin Qiao
The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques
A M Hakim
© 2022 Meta ULC. All rights reserved