Arthritis and cannabinoids: HU-210 and Win-55,212-2 prevent IL-1alpha-induced matrix degradation in bovine articular chondrocytes in-vitro.

The Journal of Pharmacy and Pharmacology
Estery C MbvundulaK D Rainsford

Abstract

Cannabinoids have analgesic, immunomodulatory and anti-inflammatory properties and attenuate joint damage in animal models of arthritis. In this study the mechanisms of action of the synthetic cannabinoid agonists, HU-210 and Win-55,212-2, were studied to determine if they affected interleukin-1 alpha (IL-1alpha)-induced proteoglycan and collagen degradation in bovine nasal cartilage explant cultures and prostaglandin E2 (PGE2) production in primary cultures of bovine articular chondrocytes. The effects of the inactive enantiomer, Win-55,212-3, were compared with those of the active enantiomer, Win-55,212-2, to determine if the effects were cannabinoid (CB)-receptor mediated. The chondrocytes and explants were stimulated by IL-1alpha (100 U mL(-1) identical with 0.06 nM and 500 U mL(-1) identical with 0.3 nM, respectively). Proteoglycan breakdown was determined as sulfated glycosaminoglycan (sGAG) release using the dimethylmethylene blue assay. Collagen degradation was determined as hydroxyproline in the conditioned culture media and cartilage digests. PGE2 was determined by ELISA. Expression of cannabinoid receptors, CB1 and CB2; cyclooxygenase-1 and -2 (COX-1 and COX-2); inducible nitric oxide synthase (iNOS); as well as acti...Continue Reading

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Citations

May 11, 2010·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Feng-Lai YuanTeng-Yue Zhang
Sep 14, 2006·Pharmacological Reviews·Pál PacherGeorge Kunos
Jun 15, 2007·Arthritis Research & Therapy·Andy Dray, Simon J Read
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May 26, 2021·Cannabis and Cannabinoid Research·Xandy Melissa Rodríguez MesaSandra Paola Santander González

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