Artificial 3D Culture Systems for T Cell Expansion

ACS Omega
Eduardo Pérez Del RíoJudith Guasch

Abstract

Adoptive cell therapy, i.e., the extraction, manipulation, and administration of ex vivo generated autologous T cells to patients, is an emerging alternative to regular procedures in cancer treatment. Nevertheless, these personalized treatments require laborious and expensive laboratory procedures that should be alleviated to enable their incorporation into the clinics. With the objective to improve the ex vivo expansion of large amount of specific T cells, we propose the use of three-dimensional (3D) structures during their activation with artificial antigen-presenting cells, thus resembling the natural environment of the secondary lymphoid organs. Thus, the activation, proliferation, and differentiation of T cells have been analyzed when cultured in the presence of two 3D systems, Matrigel and a 3D polystyrene scaffold, showing an increase in cell proliferation compared to standard suspension systems.

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Citations

Jun 20, 2019·Frontiers in Bioengineering and Biotechnology·Hasan UludagAysha Ansari
Aug 24, 2020·Biomaterials·Eduardo Pérez Del RíoJudith Guasch
Aug 10, 2019·Experimental Cell Research·Maddaly RaviAastha Joshipura
Dec 22, 2020·Biofabrication·Zhizhong JinAnhua Wu
Jan 19, 2021·ACS Biomaterials Science & Engineering·Anisha B SuraiyaAnn P Chidgey

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
flow cytometry
density gradient centrifugation

Software Mentioned

FiJi
FlowJo
ACT
Origin
Matrigel
ImageJ

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