PMID: 6162479Jan 6, 1981Paper

Artificial antigens. Antibody preparations for the localization of Lewis determinants in tissues

Biochemistry
R U LemieuxC M Switzer

Abstract

High-titer antisera specific for the Lewis-a, -b, and -d determinants were obtained by the immunization of rabbits with artificial antigens prepared by coupling chemically synthesized haptens to bovine serum albumin. The use of the appropriate synthetic immunoadsorbents allowed the isolation of the desired antibodies from the sera and the elimination of cross-reactive antibody populations. The resulting refined antibodies proved highly effective for the localization by immunofluorescence staining of Lewis antigens in epithelial cells of gastric and duodenal tissue of H patients. Furthermore, the immunohistochemical procedures could be performed in conventionally prepared paraffin tissue sections. In erythrocyte Lewis-a and -b individuals, the strongest staining reactions were with the appropriate reagent. Lewis-a individuals were Lewis-d negative. Tissue from Lewis-a--b--secretors showed strong positivity in the epithelial cells of the stomach only with the Lewis-d reagent while none of the reagents showed strong reactions with the tissue from the one nonsecretor Lewis-a--b--patient. The significance of these results to the biogenesis of the Lewis antigens is discussed.

References

Jul 9, 1975·Journal of the American Chemical Society·R U LemieuxD A Baker
May 1, 1977·Canadian Journal of Biochemistry·R U LemieuxD R Bundle
Mar 1, 1978·European Journal of Biochemistry·R ProhaskaH Tuppy
Sep 1, 1979·Clinical Immunology and Immunopathology·G K Schwarting, D M Marcus
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Aug 1, 1964·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S P KENT

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Citations

Nov 1, 1996·Bioorganic & Medicinal Chemistry·O Hindsgaul, D R Bundle
Jan 17, 2014·Journal of Medicinal Chemistry·Trudy A JacksonFrance-Isabelle Auzanneau
Mar 16, 2012·Future Medicinal Chemistry·Rachel Hevey, Chang-Chun Ling
Aug 1, 1991·Japanese Journal of Medical Science & Biology·O HosomiA Takeya
Jun 1, 1992·Journal of Biochemical and Biophysical Methods·P SantambienE Boschetti
Jul 18, 2009·Molecular Immunology·Margaret E HuflejtNicolai Bovin
Aug 15, 1984·Archives of Biochemistry and Biophysics·M BlaszczykH Koprowski
Jan 1, 1986·Vox Sanguinis·R OriolR Mollicone
Feb 15, 1990·Analytical Biochemistry·S C CrawleyM M Palcic
Jul 19, 2003·Organic Letters·Liang Liao, France-Isabelle Auzanneau
Jan 1, 1992·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·M VierbuchenR Fischer

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