Artificial chimeric exosomes for anti-phagocytosis and targeted cancer therapy

Chemical Science
Kai-Long ZhangHuanghao Yang

Abstract

Development of exosome-based delivery systems is still facing some formidable challenges, including the lack of standardized isolation and purification methods, non-large-scale production and low drug-loading efficiency. Inspired by biomimetic technologies, we turned to the design of artificial chimeric exosomes (ACEs) constructed by integrating cell membrane proteins from multiple cell types into synthetic phospholipid bilayers. For benchmarking, hybrid membrane proteins derived from red blood cells (RBCs) and MCF-7 cancer cells were selected as models. The resulting ACEs were engineered much like "Emperor Qin's Terra-Cotta Warriors", simultaneously equipped with armor (anti-phagocytosis capability from RBCs) and dagger-axes (homologous targeting ability from cancer cells). ACEs demonstrated higher tumor accumulation, lower interception and better antitumor therapeutic effect than plain liposomes in vivo, alongside large-scale standardized preparation, stable structure, high drug-loading capacity and custom-tailored functionality, highlighting the suitability of ACEs as promising alternatives of exosomes in clinical applications.

References

Aug 3, 2002·Nature Reviews. Immunology·Clotilde ThérySebastian Amigorena
Jun 11, 2005·Blood Cells, Molecules & Diseases·Lionel BlancMichel Vidal
Apr 7, 2009·Journal of Immunological Methods·Hugo De La PeñaItalo A Dodi
Jun 24, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Dongmei SunHuang-Ge Zhang
Jul 6, 2010·Journal of Proteomics·Suresh MathivananRichard J Simpson
Mar 23, 2011·Nature Biotechnology·Lydia Alvarez-ErvitiMatthew J A Wood
Sep 15, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Xiaoying ZhuangHuang-Ge Zhang
May 24, 2012·Nucleic Acids Research·Jessica WahlgrenHadi Valadi
Sep 11, 2012·Biotechnology Advances·Ruenn Chai LaiSai Kiang Lim
Nov 17, 2012·Nature Protocols·Samir El-AndaloussiMatthew J A Wood
Sep 4, 2013·Molecular Neurobiology·Anuradha KalaniNeetu Tyagi
Apr 9, 2014·Cell Research·Basant Kumar ThakurDavid Lyden
Feb 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Masamitsu KanadaChristopher H Contag
Jun 19, 2015·Nature Communications·Margaret AlexanderRyan M O'Connell
Nov 3, 2015·Nature·Ayuko HoshinoDavid Lyden
Jan 5, 2016·Journal of Extracellular Vesicles·Thomas LenerBernd Giebel
May 24, 2016·Nature Materials·R MolinaroE Tasciotti
Sep 16, 2016·Advanced Materials·Huiping SunYaping Li
Jan 14, 2017·Biomaterials·Eunee KohIn-San Kim
Feb 17, 2017·Pharmacology & Therapeutics·Lucio Barile, Giuseppe Vassalli
Mar 24, 2017·Journal of the American Chemical Society·Shuo WanWeihong Tan
Aug 16, 2017·ACS Applied Materials & Interfaces·Kai-Long ZhangHuang-Hao Yang
Jan 2, 2018·Theranostics·Chuanjiang HeBen Wang
Mar 7, 2018·Bioconjugate Chemistry·Xiangzhao AiBengang Xing
Mar 8, 2018·Advanced Materials·Roberto MolinaroEnnio Tasciotti
Apr 16, 2018·Advanced Materials·Xudong ZhangZhen Gu
Aug 1, 2018·Nano Letters·Xudong ZhangZhen Gu
Aug 1, 2017·Nature Biomedical Engineering·Sander A A KooijmansRaymond M Schiffelers

❮ Previous
Next ❯

Citations

Jan 29, 2020·Journal of Cachexia, Sarcopenia and Muscle·Shuang RongWei Yang
Aug 27, 2019·Science and Technology of Advanced Materials·Tomofumi YamamotoTakahiro Ochiya
Oct 17, 2019·IUBMB Life·Soheila MohammadiHamed Mirzaei
Apr 21, 2020·Current Pharmaceutical Design·Guifang ZhaoWenliang Li
Apr 8, 2020·Precision Clinical Medicine·Qian HuTony Ye Hu
Aug 28, 2020·International Journal of Molecular Sciences·Simona VillataValentina Cauda
Feb 12, 2020·Journal of Clinical Medicine·Max TschuschkeBartosz Kempisty
Jan 8, 2020·Biomolecules·Lalithasri RamasubramanianAijun Wang
Sep 19, 2020·Journal of Extracellular Vesicles·Kristen PopowskiKe Cheng
Oct 22, 2020·International Journal of Molecular Sciences·Ancuta JurjIoana Berindan-Neagoe
Dec 2, 2020·Pharmaceutics·Laia Hernandez-OllerSimo Schwartz
Nov 16, 2020·Biomaterials·Peipei WuHui Qian
Dec 31, 2020·Clinical and Translational Medicine·Shiyu LiKongming Wu
Jan 14, 2021·Non-coding RNA·Heidi Schwarzenbach, Peter B Gahan
Feb 24, 2021·ACS Applied Materials & Interfaces·Huiying XuBang-Ce Ye
Mar 2, 2021·Advanced Materials·Xu ZhangHélder A Santos
May 23, 2020·Cancer Letters·Mahendran ChinnappanRajagopal Ramesh
Feb 21, 2021·Seminars in Cancer Biology·Migara Kavishka JayasingheMinh T N Le
Mar 23, 2021·Advanced Drug Delivery Reviews·Michaela Schulz-Siegmund, Achim Aigner
Mar 11, 2020·Biomaterials·Mei Lu, Yuanyu Huang
Apr 17, 2021·Analytical Methods : Advancing Methods and Applications·Xindi WangQiong Jia
Jun 9, 2021·Signal Transduction and Targeted Therapy·Li ChenZhiyao He
Jun 24, 2021·ACS Applied Materials & Interfaces·Huidong HuangWeihong Tan
Jun 24, 2021·Advanced Healthcare Materials·Yong-Jiang LiDa-Xiong Xiang
Aug 14, 2021·Journal of Nanobiotechnology·Yong-Jiang LiDa-Xiong Xiang
Aug 23, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Cao Dai PhungJong Oh Kim
Sep 23, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Kailong ZhangLongxin Qiu

❮ Previous
Next ❯

Methods Mentioned

BETA
transmission
dynamic light scattering
atomic force microscopy
AFM
electrophoresis
flow cytometry
fluorescence imaging
xenograft

Software Mentioned

Drug and Statistics software ( DAS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.