PMID: 8604308Feb 15, 1996Paper

Artificial linear mini-chromosomes for Trypanosoma brucei

Nucleic Acids Research
P K PatnaikG A Cross

Abstract

We have constructed artificial linear mini- chromosomes for the parasitic protozoan Trypanosoma brucei. These chromosomes exist at approx. 2 copies per cell, are indefinitely stable under selection but are lost from 50% of the transformed population in approx. 7 generations when grown in the absence of selective pressure. Consistent with results obtained earlier with natural chromosomes in T.brucei, the telomeres on these artificial chromosomes grow, adding approx. 1- 1.5 telomeric repeats per generation. The activity of a procyclic acidic repetitive protein (parp) gene promoter on these elements is unaffected by its proximity to a telomere, implying the lack of a telomere-proximal position effect (TPE) in procyclic trypanosomes. Among other things, these autonomously replicating dispensable genetic elements will provide a defined system for the study of nuclear DNA replication, karyotypic plasticity and other aspects of chromosomal behavior in this ancient eukaryotic lineage.

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Citations

Nov 6, 2003·Molecular and Biochemical Parasitology·Bill WicksteadKeith Gull
Mar 20, 2001·Molecular and Biochemical Parasitology·S AlsfordK Gull
May 14, 2003·Molecular and Biochemical Parasitology·Daniëlle te VruchteGloria Rudenko
May 9, 1998·Molecular and Biochemical Parasitology·G A CrossM Navarro
May 16, 2000·The EMBO Journal·D HornA K Ingram
Aug 26, 1999·Parasitology Today·C E Clayton
Apr 25, 2014·International Journal for Parasitology·María de Los Ángeles CurtoAlejandro G Schijman
Jan 2, 2003·Nature Reviews. Genetics·Stephen M Beverley
Apr 24, 2020·Gene·Ayça Fulya Üstüntanır Dede, Muhittin Arslanyolu

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