Artificial microRNA-mediated resistance to cucumber green mottle mosaic virus in Nicotiana benthamiana

Planta
Chaoqiong LiangLaixin Luo

Abstract

We describe a Nicotiana benthamiana system for rapid identification of artificial microRNA (amiRNA) to control cucumber green mottle mosaic virus (CGMMV) disease. Although artificial miRNA technology has been used to control other viral diseases, it has not been applied to reduce severe cucumber green mottle mosaic virus (CGMMV) disease and crop loss in the economically important cucurbits. We used our system to identify three amiRNAs targeting CGMMV RNA (amiR1-CP, amiR4-MP and amiR6-Rep) and show that their expression reduces CGMMV replication and disease in virus-infected plants. This work streamlines the process of generating amiRNA virus-resistant crops and can be broadly applied to identify active antiviral amiRNAs against a broad spectrum of viruses to control disease in diverse crops.

References

Jul 1, 1991·The Journal of General Virology·M UgakiM Nishiguchi
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 28, 2004·Biochemical and Biophysical Research Communications·Kathy Q Luo, Donald C Chang
Apr 6, 2005·Developmental Cell·Rebecca SchwabDetlef Weigel
Mar 15, 2006·The Plant Cell·Rebecca SchwabDetlef Weigel
May 4, 2006·Annual Review of Plant Biology·Matthew W Jones-RhoadesBonnie Bartel
Mar 9, 2007·Journal of Virology·Jing QuRongxiang Fang
Feb 14, 2008·The Plant Journal : for Cell and Molecular Biology·Stephan OssowskiDetlef Weigel
Mar 20, 2008·PloS One·Norman WarthmannPhilippe Hervé
Jul 7, 2009·Annual Review of Cell and Developmental Biology·Xuemei Chen
Feb 19, 2011·Biochemical and Biophysical Research Communications·Gaurav SablokCamilo López
May 25, 2011·Biochimica Et Biophysica Acta·Basel KhraiweshJianhua Zhu
Jul 22, 2011·Journal of Virology·Guillaume LafforgueSantiago F Elena
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Feng LiBarbara Baker
Jul 25, 2013·The Plant Cell·Kestrel Rogers, Xuemei Chen
Jul 16, 2014·Plant Molecular Biology·Manish TiwariPrabodh Kumar Trivedi
Feb 26, 2016·Journal of Virological Methods·Henry WagabaNigel J Taylor
Apr 27, 2017·Frontiers in Plant Science·Chao JianHuixian Zhao
Jun 8, 2017·Annual Review of Phytopathology·Aviv DombrovskyRoger A C Jones
Jul 4, 2018·Scientific Reports·Udomporn PetchthaiSek-Man Wong
Aug 3, 2018·Molecular Plant-microbe Interactions : MPMI·Alberto CarbonellJosé-Antonio Daròs

❮ Previous
Next ❯

Citations

May 30, 2020·Plants·Adriana E Cisneros, Alberto Carbonell
Sep 11, 2020·Molecular Biology Reports·Charu KulshresthaJebi Sudan

❮ Previous
Next ❯

Datasets Mentioned

BETA
AJ245440.1
AJ243353.1
and
AY866427.1

Methods Mentioned

BETA
transgenic
PCR

Software Mentioned

Quantity One Basic
Mfold
BLAST

Related Concepts

Related Feeds

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.