PMID: 7523696Nov 1, 1994Paper

Artificial mosaic protein containing antigenic epitopes of hepatitis E virus

Journal of Virology
Y E KhudyakovH A Fields

Abstract

A synthetic gene encoding an artificial polypeptide composed of antigenic epitopes of the hepatitis E virus (HEV) proteins was constructed from short oligodeoxyribonucleotides by using PCR. The polypeptide comprises a mosaic of three antigenically active dominant regions from the protein encoded by open reading frame 2 (ORF2), one antigenically active region from the protein encoded by ORF3 of the Burmese HEV strain, and one antigenically active region from the protein encoded by ORF3 of the Mexican HEV strain. The mosaic protein was expressed in Escherichia coli as a chimera with glutathione S-transferase or beta-galactosidase. Guinea pig sera containing antibodies to the corresponding HEV synthetic peptides were used to demonstrate by Western immunoblot analysis and enzyme immunoassay the presence and accessibility of all HEV-specific antigenic epitopes introduced into the mosaic protein. Both the glutathione S-transferase and beta-galactosidase hybrid proteins were analyzed by using a panel of human anti-HEV-positive and -negative sera. The data obtained strongly indicate a diagnostic potential for the mosaic protein.

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Citations

Jun 1, 1995·Journal of Medical Virology·A BarzilaiU Martinowitz
Dec 11, 2002·Journal of Gastroenterology and Hepatology·Saleem KamiliKrzysztof Krawczynski
Jan 2, 2009·Journal of Immunoassay & Immunochemistry·T I UlanovaY E Khudyakov
Oct 3, 2012·Nature Reviews. Gastroenterology & Hepatology·Rakesh Aggarwal
Sep 17, 2013·Reviews in Medical Virology·María Teresa Pérez-GraciaMaría Caridad Montalvo Villalba
May 21, 2016·Future Microbiology·M Teresa Pérez-GraciaM Luisa Mateos-Lindemann
Sep 15, 2007·Journal of Medical Virology·Adilya AlbetkovaMichael Favorov
Apr 22, 2006·Journal of Medical Virology·Joseph J AmonGregory L Armstrong
Feb 28, 2018·Journal of Medical Microbiology·Duaa W Al-SadeqGheyath K Nasrallah
Jul 27, 1999·Virology·Y E KhudyakovH A Fields
Aug 17, 1999·Journal of Clinical Microbiology·Y E KhudyakovH A Fields

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