Artificial oocyte activation in severe teratozoospermia undergoing intracytoplasmic sperm injection

Fertility and Sterility
Mohammad Hossein Nasr-EsfahaniMarzeyeh Tavalaee

Abstract

To evaluate efficiency of ionomycin on fertilization and cleavage rates, embryo development, and pregnancy rate after intracytoplasmic sperm injection (ICSI) in teratozoospermic patients. Laboratory study. Isfahan fertility and infertility Center, and Royan institute, Tehran, Iran. Eighty-seven couples with male factor etiology (severe teratozoospermia) undergoing ICSI. Ionomycin for artificial oocyte activation. After oocyte collection, the oocytes were randomly divided into two groups: control and artificial oocyte activation (AOA). The injected oocytes in the control group were cultured in G1. The remaining oocytes were chemically activated by exposure to 10 muM ionomycin for 10 minutes. Around 16 to 18 hours after ICSI, fertilization was assessed by the presence of pronuclei. The percentage of cleavage and high-quality embryos were calculated 48 and 72 hours after ICSI. Clinical pregnancy also was determined based on ultrasound observation of a fetal heart beat. There are significant differences in the mean of fertilization and cleavage rates 72 hours after ICSI between AOA and control groups. In patients who had no fertilization in the control group and all the embryos for transfer derived from the AOA group, two pregnanci...Continue Reading

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Citations

Mar 23, 2011·Journal of Assisted Reproduction and Genetics·Astrid StecherNicolas Herbert Zech
Aug 27, 2011·Zygote : the Biology of Gametes and Early Embryos·Y LiuQ Xing
Jun 23, 2012·PloS One·Filip VasilevLuigia Santella
Jul 1, 2011·Indian Journal of Urology : IJU : Journal of the Urological Society of India·Rubina MerchantGautam N Allahbadia
Nov 21, 2015·Human Reproduction Update·Patrizia RubinoPaola Piomboni
Nov 6, 2010·Reproductive Biomedicine Online·Karen VersierenPetra De Sutter
Apr 28, 2009·Fertility and Sterility·Mohammad Hossein Nasr-EsfahaniMarziyeh Tavalaee
Sep 9, 2015·Human Reproduction Update·Marc YesteKevin Coward
Mar 25, 2014·Reproductive Biomedicine Online·Frauke Vanden MeerschautPetra De Sutter
Dec 3, 2013·Cell Calcium·Queenie V NeriGianpiero D Palermo
Oct 14, 2016·Human Fertility : Journal of the British Fertility Society·Batu AydinurazOnur Kadir Erturk
Oct 16, 2016·Journal of Assisted Reproduction and Genetics·Konstantinos A EconomouDimitris Loutradis
Dec 17, 2008·International Journal of Andrology·R John AitkenRobert I McLachlan
May 27, 2017·Human Fertility : Journal of the British Fertility Society·Yadava Bapurao JeveTarek Gelbaya
Jul 17, 2014·Reproductive Sciences·Mohammad R DeemehMohammad H Nasr-Esfahani
Apr 6, 2018·Systems Biology in Reproductive Medicine·Seda Karabulutİlknur Keskin
Aug 7, 2012·Cellular Reprogramming·Mohammad Hadi SekhavatiMohammad H Nasr-Esfahani
Mar 28, 2017·Systems Biology in Reproductive Medicine·Marziyeh TavalaeeMohammad H Nasr-Esfahani
Oct 18, 2019·Frontiers in Cell and Developmental Biology·Muhammad Babar KhawarWei Li
Jan 12, 2021·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Bing SongYunxia Cao
Dec 5, 2020·Journal of Clinical Medicine·Arantxa Cardona BarberánBjörn Heindryckx
Jun 17, 2021·Biology of Reproduction·Wenfeng XiongZhugang Wang
Dec 17, 2008·Reproductive Biomedicine Online·Elke HeytensPetra De Sutter

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