Aryl acylamidase activity on acetylcholinesterase is high during early chicken brain development

The Protein Journal
R Boopathy, P G Layer

Abstract

Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are known to exhibit aryl acylamidase activities (here called AAA(AChe) and AAA(BChe), respectively), which have been suggested to be involved in developmental and pathological processes. We here have investigated the developmental profiles of both AAA(AChe) and AAA(BChe) activities along with their AChE and BChE activities from embryonic days E3 to hatching (E21) in Triton-extracted homogenates from chicken embryonic brains. AAA(AChe) follows continuously an increase that is typical for AChE expression itself, whereas AAA(BChe) was relatively high before E10 to then become negligible toward hatching. Sucrose gradient centrifugation of both homogenized and immunopurified samples from E6-E18 brains showed that all globular forms (G1, G2, G4) of AChE present AAA(AChe) activity. Interestingly, the ratio of AAA(AChe) to AChE is highest at E6, and here again higher on G1/G2- over the G4-form. Noticeably, the sensitivity of AAA(AChe) toward the specific AChE inhibitor BW284c51 at all stages is higher than that of AChE itself. These data of high ratios of AAA associated at young stages with cholinesterases strongly indicate a role of AAA in early brain development.

Citations

May 1, 2012·Applied and Environmental Microbiology·Jun ZhangShun-Peng Li
Jan 23, 2008·Biological Chemistry·María F MontenegroCecilio J Vidal
Jan 12, 2010·Behavioural Brain Research·Yael Abreu-VillaçaAlex C Manhães
Aug 11, 2007·Biochimica Et Biophysica Acta·Patrick MassonLawrence M Schopfer
Mar 24, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Rathanam BoopathyPaul G Layer
Jan 22, 2008·The FEBS Journal·Laura E Paraoanu, Paul G Layer
Jun 11, 2008·Chemico-biological Interactions·Jean MassouliéSuzanne Bon
Jan 21, 2006·Chemico-biological Interactions·Karla V AllebrandtPaul G Layer
Oct 11, 2012·Chemico-biological Interactions·Paul G LayerAstrid Vogel-Höpker
Aug 31, 2017·Molecular and Cellular Biochemistry·Raj Kumar ChinnaduraiRathanam Boopathy
May 26, 2018·Environmental Science and Pollution Research International·Caio R D AssisLuiz B Carvalho
Jul 30, 2005·Journal of Cellular Biochemistry·Matthew D ThullberyKathleen M George
Mar 26, 2020·International Immunopharmacology·Janine SpiekerPaul G Layer
Feb 21, 2021·Osteoarthritis and Cartilage·Marianne LauwersChunyi Wen

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