PMID: 241681Aug 1, 1975

Aryl hydrocarbon hydroxylase in liver nuclei of C3H/He and DBA/2 mice

Gann = Gan
M WatanabeK Konno


Aryl hydrocarbon hydroxylase activity in liver nuclei from C3H/He mice is apparently increased by the administration of 3-methylcholanthrene, but the enzyme activity from the DBA/2 mice is not. On the other hand, by treatment of the mice with phenobarbital sodium, the increased activity in liver nuclei was observed in both strains of mice. There are approximately the same levels of the apparent Km for benzo[alpha]pyrene in liver nuclei from both strains of mice even after treatment with 3-methylcholanthrene, but different Km values for NADPH and NADH are observed between the constitutive and induced enzyme, showing 0.032 and 0.091 mM for NADPH, and 0.303 and 1.67 mM for NADH, respectively. Both 5, 6- and 7, 8-benzoflavones enhance the activity in the constitutive enzyme, but inhibit it in the induced enzyme non-competitively. Nicotinamide inhibits both enzyme activities in liver nuclei. Cyclohexene oxide and 1, 1, 1-trichloropropane oxide enhance the activity in the induced enzyme, but not in the constitutive enzyme in liver nuclei. The differences in the properties between the contitutive and induced enzymes and between the enzymes in microsomes and in nuclei from mouse liver were discussed briefly.

Related Concepts

Xenobiotic Monooxygenases
Cell Nucleus
Endoplasmic Reticulum
9,10-Epoxypalmitic Acid Hydrase

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.