Aryl sulfatase inactivation of slow reacting substance. Evidence for proteolysis as a major mechanism when ordinary commercial preparations of the enzyme are used

Prostaglandins
C W ParkerS F Falkenhein

Abstract

Type II B arylsulfatases are known to inactivate slow reacting substance (SRS), but the mechanism is unclear. In the present study, ordinary commercial preparations of Sigma limpet arylsulfatase largely inactivated the glutathionyl and cysteinyl-glycyl forms of SRS, but the cysteinyl form of SRS was largely resistant to the enzyme. Evidence is presented which established that a major mechanism for the inactivation of the glutathionyl and cysteinyl-glycyl SRS types, at least by the particular enzyme preparations we have studied, involves cleavage of the glycine moiety from the sulfur containing side chain. This was confirmed by digestion studies with glutathione itself. In addition, there is some evidence to indicate that the enzyme may destabilize the double bond structure of the SRS molecule, contributing to the overall inactivation.

References

Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B A JakschikC W Parker
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R C MurphyB Samuelsson
Dec 28, 1979·Biochemical and Biophysical Research Communications·S HammarströmE J Corey
Jan 1, 1979·The Journal of Allergy and Clinical Immunology·C W Parker
Feb 1, 1979·Prostaglandins and Medicine·W E KibbeyJ P Minton
Aug 28, 1979·Biochemical and Biophysical Research Communications·C W ParkerS F Falkenhein
Nov 12, 1977·British Medical Journal·N M JohnsonS W Clarke
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L OrningB Samuelsson
May 1, 1980·Prostaglandins·B Samuelsson, S Hammarström
Jan 1, 1960·Annual Review of Biochemistry·J D GREGORY, P W ROBBINS

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Citations

Jan 1, 1992·Veterinary Research Communications·B J McEwen
Dec 16, 1980·Biochemical and Biophysical Research Communications·C W ParkerS F Falkenhein

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