PMID: 8590465Jul 1, 1995Paper

As in Saccharomyces cerevisiae, aspartate transcarbamoylase is assembled on a multifunctional protein including a dihydroorotase-like cryptic domain in Schizosaccharomyces pombe

Current Genetics
M LollierJ L Souciet

Abstract

The organisation of the URA1 gene of Schizosaccharomyces pombe was determined from the entire cDNA cloned by the transformation of an ATCase-deficient strain of Saccharomyces cerevisiae. The URA1 gene encodes the bifunctional protein GLNase/CPSase-ATCase which catalyses the first two steps of the pyrimidine biosynthesis pathway. The complete nucleotide sequence of the URA1 cDNA was elucidated and the deduced amino-acid sequence was used to define four domains in the protein; three functional domains, corresponding to GLNase (glutamine amidotransferase), CPSase (carbamoylphosphate synthetase) and ATCase (aspartate transcarbamoylase) activities, and one cryptic DHOase (dihydroorotase) domain. Genetic investigations confirmed that both GLNase/CPSase and ATCase activities are carried out by the same polypeptide. They are also both feedback-inhibited by UTP (uridine triphosphate). Its organization and regulation indicate that the S. pombe URA1 gene product appears very similar to the S. cerevisiae URA2 gene product.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
May 1, 1992·The Plant Journal : for Cell and Molecular Biology·M MinetF Lacroute
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M NagyD Thomas
Jan 1, 1991·Methods in Enzymology·D M Becker, L Guarente
Jan 1, 1991·Methods in Enzymology·F Sherman, J Hicks
Sep 1, 1987·Molecular & General Genetics : MGG·S PotierF Lacroute
Jan 5, 1987·Journal of Molecular Biology·J N Freund, B P Jarry
Apr 1, 1988·Molecular & General Genetics : MGG·A GuyonvarchF Lacroute
Jul 11, 1988·Nucleic Acids Research·W J DowerC W Ragsdale
Sep 5, 1985·Journal of Molecular Biology·R LossonF Lacroute
Apr 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·D B Clewell, D R Helinski
Dec 1, 1969·Analytical Biochemistry·L M Prescott, M E Jones
Mar 1, 1968·Journal of Bacteriology·F Lacroute
Jul 1, 1965·Journal of General Microbiology·F LacrouteJ M Wiame
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J Sakaguchi, M Yamamoto
Mar 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J N DavidsonC B Kern
Feb 1, 1984·Current Genetics·A Gygax, P Thuriaux

❮ Previous
Next ❯

Citations

Oct 19, 2000·Research in Microbiology·J R JohnstonR K Mortimer
Aug 15, 2015·International Journal of Molecular Sciences·Dashuang ShiMendel Tuchman
Sep 24, 2004·The Journal of Biological Chemistry·Anupama AhujaDavid R Evans
Nov 13, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Nada LallousSantiago Ramón-Maiques

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.