Asbestos and multi-walled carbon nanotubes generate distinct oxidative responses in inflammatory cells

Journal of Clinical Biochemistry and Nutrition
Satomi FunahashiShinya Toyokuni

Abstract

Asbestos exposure is considered a social burden by causing mesothelioma. Despite the use of synthetic materials, multi-walled carbon nanotubes (MWCNTs) are similar in dimension to asbestos and produce mesothelioma in animals. The role of inflammatory cells in mesothelial carcinogenesis remains unclear. Here, we evaluated the differences in inflammatory cell responses following exposure to these fibrous materials using a luminometer and L-012 (8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4-(2H,3H) dione) to detect reactive oxygen species (ROS). Rat peripheral blood or RAW264.7 cells were used to assess the effects on neutrophils and macrophages, respectively. Crocidolite and amosite induced significant ROS generation by neutrophils with a peak at 10 min, whereas that of chrysotile was ~25% of the crocidolite/amosite response. MWCNTs with different diameters (~15, 50, 115 and 145 nm) and different carcinogenicity did not induce significant ROS in peripheral blood. However, the MWCNTs induced a comparable amount of ROS in RAW264.7 cells to that following asbestos treatment. The peaks for MWCNTs (0.5-1.5 h) were observed earlier than those for asbestos (1-5 h). Apocynin and superoxide dismutase significantly inhibited ROS gen...Continue Reading

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Citations

Dec 20, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·T I VitkinaK S Golokhvast
Oct 16, 2016·Archives of Toxicology·Andrea Haase, Andreas Luch
Aug 3, 2017·Environmental Science and Pollution Research International·Tingting HuoDongping Sun
Jun 11, 2019·Genes and Environment : the Official Journal of the Japanese Environmental Mutagen Society·Takenori IshidaAkio Kuroda
Jun 21, 2020·International Journal of Environmental Research and Public Health·Rachel LamAnna Nolan

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Methods Mentioned

BETA
electron spin resonance

Software Mentioned

GraphPad
Prism5

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