ASC-particle-induced Peritonitis

Bio-protocol
Lucia de AlmeidaChristian Stehlik

Abstract

In response to pathogen infection and tissue damage, inflammasome sensors such as NLRP3 and AIM2 are activated, which triggers PYRIN domain (PYD)-mediated ASC nucleation, followed by self-perpetuating ASC polymerization, which ultimately culminates in caspase-1 activation, interleukin (IL)-1β and IL-18 processing and release and pyroptosis (Ratsimandresy et al., 2013; Cai et al., 2014). Inflammasomes release not only cytokines, but also the polymeric ASC danger particles (pASC) by pyroptosis, which perpetuate and propagate inflammasome responses to bystander cells to engage cell intrinsic ASC and caspase-1 (Baroja-Mazo et al., 2014; Franklin et al., 2014). In this protocol we describe intraperitoneal injection of polymeric ASC particles as a danger signal and measure neutrophil infiltration and levels of the pro-inflammatory cytokine IL-1β by ELISA in the peritoneal lavage (de Almeida et al., 2015).

References

Jul 30, 2008·Methods in Enzymology·Teresa Fernandes-Alnemri, Emad S Alnemri
Dec 25, 2013·Frontiers in Immunology·Rojo A RatsimandresyChristian Stehlik
Jun 24, 2014·Nature Immunology·Bernardo S FranklinEicke Latz
May 20, 2015·Bio-protocol·Fátima Martín-SánchezPablo Pelegrín

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Citations

Dec 17, 2017·Immunological Reviews·Bernardo S FranklinFlorian Ingo Schmidt

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