Asc1, homolog of human RACK1, prevents frameshifting in yeast by ribosomes stalled at CGA codon repeats

RNA
Andrew S Wolf, Elizabeth J Grayhack

Abstract

Quality control systems monitor and stop translation at some ribosomal stalls, but it is unknown if halting translation at such stalls actually prevents synthesis of abnormal polypeptides. In yeast, ribosome stalling occurs at Arg CGA codon repeats, with even two consecutive CGA codons able to reduce translation by up to 50%. The conserved eukaryotic Asc1 protein limits translation through internal Arg CGA codon repeats. We show that, in the absence of Asc1 protein, ribosomes continue translating at CGA codons, but undergo substantial frameshifting with dramatically higher levels of frameshifting occurring with additional repeats of CGA codons. Frameshifting depends upon the slow or inefficient decoding of these codons, since frameshifting is suppressed by increased expression of the native tRNA(Arg(ICG)) that decodes CGA codons by wobble decoding. Moreover, the extent of frameshifting is modulated by the position of the CGA codon repeat relative to the translation start site. Thus, translation fidelity depends upon Asc1-mediated quality control.

References

Jan 1, 1992·Annual Review of Genetics·C G Kurland
Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·C TuJ A Bruenn
May 30, 2001·The International Journal of Biochemistry & Cell Biology·G KramerB Hardesty
Jan 20, 2004·RNA·Pavel V BaranovJohn F Atkins
Jul 9, 2004·Molecular & Cellular Proteomics : MCP·Andrei AlexandrovElizabeth J Grayhack
Aug 31, 2004·Nature Structural & Molecular Biology·Jayati SenguptaJoachim Frank
Nov 24, 2004·Nature Structural & Molecular Biology·Frank V Murphy, V Ramakrishnan
Dec 3, 2005·Genes & Development·Daniel M GelperinElizabeth J Grayhack
Feb 13, 2007·Annual Review of Biochemistry·Sean D Moore, Robert T Sauer
Jul 31, 2007·Genetics·Marenda A WilsonAmbro van Hoof
Aug 3, 2007·Genes & Development·Olaf Isken, Lynne E Maquat
Dec 17, 2008·Journal of Proteomics·Astrid BruckmannG Paul H van Heusden
Dec 31, 2008·Molecular and Cellular Biology·Scott M CoyleJennifer A Doudna
Feb 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jessie ChuSteve A Kay
Feb 9, 2010·Nature Structural & Molecular Biology·Shashi BhushanRoland Beckmann
Sep 14, 2010·Nature·Mario H Bengtson, Claudio A P Joazeiro
Sep 30, 2010·Molecular and Cellular Biology·Arpita BhattacharyaJonathan R Warner
Oct 5, 2010·Nature Structural & Molecular Biology·Liming ChenHaiwei Song
Oct 26, 2010·RNA·Daniel P LetzringElizabeth J Grayhack
Jul 16, 2011·The Journal of Biological Chemistry·Jason S SeidmanChristopher S Hayes
Oct 8, 2011·Cell Communication and Signaling : CCS·David R AdamsPatrick A Kiely
Dec 7, 2011·Proceedings of the National Academy of Sciences of the United States of America·Christopher J Shoemaker, Rachel Green
Jun 6, 2012·Nature Structural & Molecular Biology·Christopher J Shoemaker, Rachel Green
Jun 21, 2012·Wiley Interdisciplinary Reviews. RNA·Jonathan D Dinman
Oct 9, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ying FangAndrew E Firth
Oct 17, 2012·Molecular & Cellular Proteomics : MCP·Nicole RachfallGerhard H Braus
Feb 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Christopher J WoolstenhulmeAllen R Buskirk
Jun 4, 2013·Molecular Cell·Erik GutierrezThomas E Dever

❮ Previous
Next ❯

Citations

Jul 5, 2016·Cell·Caitlin E GambleElizabeth J Grayhack
Aug 21, 2016·FEBS Letters·Ken IkeuchiToshifumi Inada
Oct 18, 2016·Trends in Biochemical Sciences·Toshifumi Inada
Mar 16, 2017·Trends in Genetics : TIG·Christina E Brule, Elizabeth J Grayhack
Sep 9, 2017·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Didier Auboeuf
Jun 6, 2018·Wiley Interdisciplinary Reviews. RNA·Laura L Arthur, Sergej Djuranovic
Nov 9, 2016·Molecular and Cellular Biology·Kerstin SchmittOliver Valerius
Sep 12, 2018·Molecular and Cellular Biology·Simone GalloStefano Biffo
Dec 7, 2018·The Journal of Biological Chemistry·Kevin C Stein, Judith Frydman
Nov 2, 2018·Wiley Interdisciplinary Reviews. RNA·Nathan MeadeDerek Walsh
May 30, 2020·ELife·Slavica Pavlovic DjuranovicSergej Djuranovic
Jun 23, 2020·Annual Review of Biochemistry·Cole S Sitron, Onn Brandman
Jul 15, 2020·Biochemical Society Transactions·Shir Bahiri-Elitzur, Tamir Tuller
Sep 23, 2016·BioMed Research International·Xue ZhangGuotian Li
Feb 15, 2017·RNA·Nicholas R Guydosh, Rachel Green
Jul 19, 2017·Annual Review of Cell and Developmental Biology·Claudio A P Joazeiro
Feb 6, 2019·Nature Communications·Marie-Luise WinzDavid Tollervey
Sep 29, 2017·RNA·Kelly A LimoncelliMelissa J Moore
Oct 27, 2017·FEMS Microbiology Reviews·Jürgen J Heinisch, Rosaura Rodicio
Aug 2, 2017·Nature Communications·Yoshitaka MatsuoToshifumi Inada
Aug 4, 2020·ELife·Niladri K SinhaRachel Green
Aug 17, 2020·The Biochemical Journal·Rodrigo D RequiãoFernando L Palhano
Aug 15, 2019·Cell Reports·Carrie L SimmsHani S Zaher
Jan 20, 2021·Cell Death Discovery·Francesco MonticoloMaria Luisa Chiusano
Jul 3, 2020·Molecular Cell·Sezen Meydan, Nicholas R Guydosh
Nov 14, 2020·ELife·Markus TerreySusan L Ackerman
Apr 2, 2021·Proceedings of the National Academy of Sciences of the United States of America·Agnieszka A PochopienDaniel N Wilson
Mar 29, 2021·The Journal of Biological Chemistry·Géssica C BarrosFernando L Palhano
Aug 1, 2021·Nature Communications·Gabriel DemoAndrei A Korostelev

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved