Ascertaining effects of nanoscale polymeric interfaces on competitive protein adsorption at the individual protein level

Nanoscale
Sheng SongJong-In Hahm

Abstract

With the recent development of biomaterials and biodevices with reduced dimensionality, it is critical to comprehend protein adhesion processes to nanoscale solid surfaces, especially those occurring in a competitive adsorption environment. Complex sequences of adhesion events in competitive adsorption involving multicomponent protein systems have been extensively investigated, but our understanding is still limited primarily to macroscopic adhesion onto chemically simple surfaces. We examine the competitive adsorption behavior from a binary protein mixture containing bovine serum albumin and fibrinogen at the single protein level. We subsequently evaluate a series of adsorption and displacement processes occurring on both the macroscopic homopolymer and nanoscopic diblock copolymer surfaces, while systematically varying the protein concentration and incubation time. We identify the similarities and dissimilarities in competitive protein adsorption behavior between the two polymeric surfaces, the former presenting chemical uniformity at macroscale versus the latter exhibiting periodic nanointerfaces of chemically alternating polymeric segments. We then present our novel experimental finding of a large increase in the nanointerf...Continue Reading

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Citations

Mar 1, 2018·Annual Review of Physical Chemistry·Nicholas A MoringoChristy F Landes
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Jun 9, 2021·The Journal of Physical Chemistry. B·David H Cho, Jong-In Hahm
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Nov 16, 2018·ACS Applied Materials & Interfaces·Ruiqing HouFrank Feyerabend
Feb 23, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Nicholas A MoringoChristy F Landes

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