Ascites-Derived Extracellular microRNAs as Potential Biomarkers for Ovarian Cancer

Reproductive Sciences
Luděk ZáveskýAleš Hořínek

Abstract

Ovarian cancer as the most fatal gynecological malignancy is often manifested by excessive fluid accumulation known as ascites or effusion. Ascites-derived microRNAs (miRNAs) may be closely associated with ovarian cancer progression. However, our knowledge of their roles, altered expression, and clinical outcomes remained limited. In this study, large-scale expression profiling of 754 human miRNAs was performed using real-time quantitative polymerase chain reaction and 384-well TaqMan array human miRNA A and B cards to identify differentially expressed miRNAs between extracellular fraction of the ascitic fluid associated with high-grade serous ovarian carcinomas and control plasma. Of the 754 miRNAs, 153 were significantly differentially expressed relative to the controls. Expression of 7 individual miRNAs (miR-200a, miR-200b, miR-200c, miR-141, miR-429, miR-1290, and miR-30a-5p) was further validated in extended sample sets, including serous, endometrioid, and mucinous subtypes. All miR-200 family members and miR-1290 were conspicuously overexpressed, while miR-30a-5p was only weakly overexpressed. The ability of miRNAs expression to discriminate the pathological samples from the controls was strong. Receiver operating charact...Continue Reading

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Citations

Mar 16, 2019·Reproductive Sciences·Maria Rosa Maduro
Jun 4, 2019·Cancer Cytopathology·Lorenzo NicolèAmbrogio Fassina
Apr 2, 2019·Experimental and Therapeutic Medicine·Mitsuru ChibaHaruka Kuwata
Sep 29, 2019·Molecular Aspects of Medicine·Guo-Liang HuangGeorge A Calin

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