Ascitic fluid regulates the local innate immune response of patients with cirrhosis

Journal of Leukocyte Biology
Juan Camilo NietoSilvia Vidal

Abstract

Ascitic neutrophils from cirrhotic patients with spontaneous bacterial peritonitis (SBP) exhibit an impaired oxidative burst that could facilitate bacterial infection. However, the influence of the cell-free ascitic fluid of these patients on neutrophil function has not been investigated. To analyze this influence, we determined the ascitic levels of cytokines, resistin, and lactoferrin and their association with neutrophil function, disease severity score, and SBP resolution. We analyzed NETosis induction by microscopy and oxidative burst by the flow cytometry of healthy neutrophils cultured in ascitic fluid from cirrhotic patients with sterile ascites (SA) and with SBP before and after antibiotic treatment. Resistin, IL-6, IL-1 receptor antagonist, IL-1β, and lactoferrin levels were measured in ascitic fluids and supernatants of cultured neutrophils and PBMCs by ELISA. Upon stimulation, healthy neutrophils cultured in SBP ascitic fluid produced lower NETosis and oxidative burst than those cultured in SA. Ascitic resistin levels were negatively correlated with NETosis, oxidative burst, and ascitic glucose levels; and positively correlated with the model for end-stage liver disease score. After an E. coli or TNF-α stimulus, neu...Continue Reading

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Citations

Sep 19, 2018·Scandinavian Journal of Immunology·Juan Manuel Agraz-CibriánMary Fafutis-Morris
Apr 20, 2021·Frontiers in Immunology·Arjuna Singanayagam, Evangelos Triantafyllou
May 12, 2021·Antimicrobial Agents and Chemotherapy·René WelteRomuald Bellmann
Jul 6, 2021·Frontiers in Immunology·Yanran LiZhicai Zuo

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