Ascl1 Coordinately Regulates Gene Expression and the Chromatin Landscape during Neurogenesis

Cell Reports
Alexandre A S F RaposoDiogo Castro

Abstract

The proneural transcription factor Ascl1 coordinates gene expression in both proliferating and differentiating progenitors along the neuronal lineage. Here, we used a cellular model of neurogenesis to investigate how Ascl1 interacts with the chromatin landscape to regulate gene expression when promoting neuronal differentiation. We find that Ascl1 binding occurs mostly at distal enhancers and is associated with activation of gene transcription. Surprisingly, the accessibility of Ascl1 to its binding sites in neural stem/progenitor cells remains largely unchanged throughout their differentiation, as Ascl1 targets regions of both readily accessible and closed chromatin in proliferating cells. Moreover, binding of Ascl1 often precedes an increase in chromatin accessibility and the appearance of new regions of open chromatin, associated with de novo gene expression during differentiation. Our results reveal a function of Ascl1 in promoting chromatin accessibility during neurogenesis, linking the chromatin landscape at Ascl1 target regions with the temporal progression of its transcriptional program.

Associated Datasets

Feb 16, 2015·Diogo S CastroDiogo S Castro

References

Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K Roemer, T Friedmann
Jul 3, 2002·Nature Reviews. Neuroscience·Nicolas BertrandFrançois Guillemot
Jul 31, 2003·Trends in Cell Biology·Marianna B Ruzinova, Robert Benezra
Sep 16, 2003·Molecular and Cellular Biology·Michel-Olivier GrattonStefano Stifani
May 20, 2004·Journal of Cell Science·Gabor ForgacsChristopher G Burd
Jun 25, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ryosuke OhsawaRyoichiro Kageyama
Aug 10, 2005·PLoS Biology·Luciano ContiAustin Smith
Jun 13, 2006·Cerebral Cortex·Steven M PollardAustin Smith
Jun 15, 2007·The European Journal of Neuroscience·Benedikt BerningerMagdalena Götz
Aug 10, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Benedikt BerningerMagdalena Götz
Sep 19, 2008·Genome Biology·Yong ZhangX Shirley Liu
Feb 12, 2009·Journal of Cell Science·Rubén Alvarez-Rodríguez, Sebastián Pons
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
Jun 27, 2009·Annual Review of Neuroscience·Arnold Kriegstein, Arturo Alvarez-Buylla
Sep 9, 2009·Nature Reviews. Genetics·Peter J Park
Mar 11, 2010·Neuroscience Letters·Joshua D AakerNaoko Koyano-Nakagawa
Jan 5, 2011·PLoS Biology·Valeria KalteziotiPanagiotis K Politis
Nov 8, 2011·Genes & Development·Kenneth S Zaret, Jason S Carroll
Mar 1, 2012·Nature Methods·Jason Ernst, Manolis Kellis
Sep 8, 2012·Genome Research·Anirudh NatarajanUwe Ohler
Sep 4, 2013·Neuroscience·G WilkinsonC Schuurmans
Dec 19, 2014·Frontiers in Cellular Neuroscience·Francisca F Vasconcelos, Diogo S Castro

❮ Previous
Next ❯

Citations

Jul 28, 2016·Cell Discovery·Sudhir ThakurelaVijay K Tiwari
Oct 21, 2015·Proceedings of the National Academy of Sciences of the United States of America·Yumi UekiThomas A Reh
Oct 11, 2015·Developmental Biology·Shiqi JiaCarmen Birchmeier
Aug 22, 2015·Current Opinion in Genetics & Development·Sophie Péron, Benedikt Berninger
May 6, 2016·Neuroscience Bulletin·Fengyun ZhangDongya Zhu
Oct 30, 2016·Acta Biochimica Et Biophysica Sinica·Zheying MinQinghua Tao
Oct 6, 2016·Cell Reports·Francisca F VasconcelosDiogo S Castro
Jun 26, 2017·Biochemical Pharmacology·Enrica BodaAnnalisa Buffo
Feb 14, 2018·Nucleic Acids Research·Raquel FueyoMarian A Martínez-Balbás
Aug 9, 2018·Nature Communications·Julien BryoisGregory E Crawford
Mar 7, 2018·The Journal of Biological Chemistry·Alexandre Mayran, Jacques Drouin
Sep 28, 2017·Molecular Psychiatry·L HuangM F Wilkinson
Mar 17, 2018·Scientific Reports·Sébastien GillotinAnna Philpott
Aug 22, 2017·Frontiers in Cellular Neuroscience·Vinicius T Ribas, Marcos R Costa
Apr 16, 2016·Oncotarget·Guillaume BrocquevilleRoland P Bourette
May 28, 2020·Glia·Carlos ParrasQing Richard Lu
Aug 4, 2019·Nature Communications·Lydie CouturierFrançois Schweisguth
Aug 16, 2018·Proceedings of the National Academy of Sciences of the United States of America·Corentine MarieCarlos Parras
Dec 14, 2018·Frontiers in Bioengineering and Biotechnology·Meghan RobinsonStephanie M Willerth
Jul 22, 2019·Nature Communications·Xu-Dong WangYonghao Yu
Oct 28, 2019·International Journal of Molecular Sciences·Aneta Tarczewska, Beata Greb-Markiewicz
Mar 7, 2017·Frontiers in Cell and Developmental Biology·Britta M Grebbin, Dorothea Schulte
Sep 21, 2017·Nature Communications·Alessandra ZappuloMarina Chekulaeva
Jun 20, 2018·Nature Neuroscience·Marisa KarowBenedikt Berninger
Nov 30, 2018·Frontiers in Cellular Neuroscience·Roberta Pereira de Melo GuimarãesMarcos R Costa
May 16, 2018·Development·Roberta AzzarelliAnna Philpott
May 6, 2017·Frontiers in Biology·Fatih Semerci, Mirjana Maletic-Savatic
Dec 28, 2018·Nucleic Acids Research·Camilla Ciolli MattioliMarina Chekulaeva

❮ Previous
Next ❯

Methods Mentioned

BETA
immunoprecipitation
nuclear translocation
ChIP
ChIP-PCR
ChIP-seq
Footprinting
FAIRE-PCR
Infection
PCR
Assay

Software Mentioned

BeedStudio
ChromHMM
Digital Genomic Footprinting ( DGF
Bowtie
MACS
GeneSpring X

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.