Apr 8, 1976

Ascorbic acid-2-sulfate sulfhohydrolase activity of human arylsulfatase A

Biochimica Et Biophysica Acta
A L FluhartyH Kihara


Pure human arylsulfatase A (EC was found to hydrolyze ascorbic acid 2-sulfate to ascorbic acid and inorganic sulfate at rates from 200 to 2000 mumol/mg per h depending on the method of assay. This rate was lower than that observed with the synthetic substrate 4-nitrocatechol sulfate, but higher than that seen with the physiological substrate cerebroside sulfate. Extracts of cultured fibroblasts from normal subjects were also shown to hydrolyze ascorbic acid 2-sulfate; extracts of fibroblasts from patients with metachromatic leukodystrophy, known to be deficient in arylsulfatase A, did not. Similarly, hydrolysis of ascorbic acid 2-sulfate was not observed when a partially purified preparation of human arylsulfatase B was tested under a variety of conditions. Thus, in the human, arylsulfatase A appears to be the major, if not the only, ascorbic acid-2-sulfate sulfohydrolase.

  • References8
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Mentioned in this Paper

ARSB gene
Specimen Type - Fibroblasts
Leukodystrophy, Metachromatic
Structure-Activity Relationship
Ascorbic acid 2-sulfate
Ascorbic Acid Measurement
4-nitrocatechol sulfate

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